Analysis of the expression pattern of the schizophrenia-risk and intellectual disability gene TCF4 in the developing and adult brain suggests a role in development and plasticity of cortical and hippocampal neurons
- Matthias Jung†1,
- Benjamin M. Häberle†1,
- Tristan Tschaikowsky1,
- Marie-Theres Wittmann1, 2,
- Elli-Anna Balta1,
- Vivien-Charlott Stadler1,
- Christiane Zweier2,
- Arnd Dörfler3,
- Christian Johannes Gloeckner4, 5 and
- D. Chichung Lie1Email author
© The Author(s). 2018
Received: 7 September 2017
Accepted: 20 February 2018
Published: 22 March 2018
Haploinsufficiency of the class I bHLH transcription factor TCF4 causes Pitt-Hopkins syndrome (PTHS), a severe neurodevelopmental disorder, while common variants in the TCF4 gene have been identified as susceptibility factors for schizophrenia. It remains largely unknown, which brain regions are dependent on TCF4 for their development and function.
We systematically analyzed the expression pattern of TCF4 in the developing and adult mouse brain. We used immunofluorescent staining to identify candidate regions whose development and function depend on TCF4. In addition, we determined TCF4 expression in the developing rhesus monkey brain and in the developing and adult human brain through analysis of transcriptomic datasets and compared the expression pattern between species. Finally, we morphometrically and histologically analyzed selected brain structures in Tcf4-haploinsufficient mice and compared our morphometric findings to neuroanatomical findings in PTHS patients.
TCF4 is broadly expressed in cortical and subcortical structures in the developing and adult mouse brain. The TCF4 expression pattern was highly similar between humans, rhesus monkeys, and mice. Moreover, Tcf4 haploinsufficiency in mice replicated structural brain anomalies observed in PTHS patients.
Our data suggests that TCF4 is involved in the development and function of multiple brain regions and indicates that its regulation is evolutionary conserved. Moreover, our data validate Tcf4-haploinsufficient mice as a model to study the neurodevelopmental basis of PTHS.
The transcription factor 4 (TCF4, Gene ID: 6925) and its paralogues TCF3 and TCF12 form the subgroup of class I basic Helix-Loop-Helix (bHLH) transcription factors (TFs). Class I bHLH TFs are ubiquitously expressed, and their transcriptional output is dependent on their interaction partners. They are obligate partners for proneural class II bHLH TFs, which are unable to exert transcriptional function without dimerization to Class I bHLH. Class I bHLH TF are inhibited by interaction Class V bHLH factors, which do not possess a DNA-binding domain and sequester class I factors .
TCF4 has gained major attention following the discoveries that heterozygote loss-of-function mutations in TCF4 cause Pitt-Hopkins syndrome (PTHS) [2, 3]—a neurodevelopmental disorder characterized by early onset developmental delay, moderate to severe intellectual disability, autistic behavior, intermittent breathing abnormalities, seizures, and distinctive facial features—and that single nucleotide polymorphisms (SNPs) in potentially regulatory regions of TCF4 are associated with an increased risk of schizophrenia [4–6].
The extent of cognitive deficits in PTHS suggests that TCF4 dosage is critical for the development of various brain regions, but it remains largely unknown which neural populations develop in a TCF4-dependent fashion. Based on the rationale that TCF4 expression will predict regions that require TCF4 activity for their development, we performed a systematic analysis of the temporo-spatial expression pattern of TCF4 protein in mouse brain development. Moreover, we determined TCF4 expression in the adult mouse brain to identify neural systems, in which TCF4 may serve functions in maintenance and plasticity. Our analyses reveal that TCF4 is broadly expressed in cortical and subcortical structures in the development and adulthood with prominent expression in the cortex, hippocampus, and a subset of hypothalamic and amygdaloid nuclei. Comparison of the developmental and adult murine expression pattern with transcriptomic datasets from rhesus monkey and human brain revealed strong similarities in TCF4 expression between species. Moreover, we found that Tcf4 haploinsuffiency in mice replicated structural brain anomalies that have been observed in individuals with PTHS. Thus, our data suggest that TCF4 regulation and function in brain development may be evolutionary conserved.
Next, we systematically analyzed the expression pattern of TCF4 protein in the developing and adult mouse brain. On embryonic day 11.5 (E11.5), TCF4 was broadly expressed in the germinal regions for cortical glutamatergic neurons and GABAergic neurons. Immunoreactive intensity varied between the distinct germinal regions. It was high in the cortical and hippocampal neuroepithelium and remained intense in the expanding neocortex and in the developing hippocampal formation (Fig. 1d–h). The ganglionic eminences are the main germinal zones for GABAergic neurons. The lateral ganglionic eminence (LGE) generates GABAergic interneurons and projection neurons of the olfactory bulb, amygdala, and striatum from E12 until birth [7, 8], while the medial ganglionic eminence (MGE) generates the majority of cortical interneurons between E12 and E16.5. MGE-derived interneuron precursors migrate tangentially to their final neocortical location . Notably, the expression of TCF4 in the MGE correlated with the time of cortical interneuron production  (Fig. 1d–f). Moreover, TCF4 expression showed a lateral to medial gradient with moderate to high levels in the most medial part of the MGE and low to absent immunoreactivity in the LGE (Fig. 1d–f).
Next, we analyzed the expression of TCF4 in glial cells. TCF4 was expressed in the majority of astroglia and some Sox10-positive oligodendroglia (Fig. 4F–H”). Expression levels in both glial populations varied from low to high. Iba1-positive microglial cells did not show TCF4 immunoreactivity (Fig. 4F–I”).
Next, we sought to evaluate whether the TCF4 expression pattern is conserved from mice to humans. The NIH Blueprint Non-Human Primate (NHP) Atlas (2009) (http://www.blueprintnhpatlas.org) provides mRNA expression data of the developing rhesus monkey brain from E40–0 months; the BrainSpan Atlas documents mRNA expression data of human neural development for post conception weeks 15–21 . TCF4 mRNA expression data in the adult human brain was extracted from the Allen Human Brain Atlas (© 2010 Allen Institute for Brain Science. Allen Human Brain Atlas. Available from: human.brain-map.org, ). The neurodevelopmental expression pattern of TCF4 was highly similar between mice, rhesus monkeys, and humans: regions with high expression during murine neural development such as the hippocampus, the cortex, the ganglionic eminences, and some nuclei of the amygdaloid complex, consistently show high TCF4 expression in the developing brain of rhesus monkey and humans, whereas low TCF4 expressing regions such as the caudoputamen also show low expression during rhesus monkey and human brain development (Fig. 3a-c).
Striking similarities in the TCF4 expression pattern were also found during adulthood. Regions with high expression in adult mice, i.e., hippocampus, cerebellum, cortex, and nuclei of the amygdaloid complex, were also the highest TCF4-expressing regions in the adult human brain, while, for example, the diencephalon, metencephalon, myelencephalon, and parts of the basal ganglia showed low TCF4 expression levels in humans and mice (Fig. 3d, e). These data indicate that the expression pattern of TCF4 is conserved from mouse to humans.
We next investigated whether Tcf4 haploinsufficiency in mice reproduced structural anomalies of PTHS. To determine the influence of Tcf4-haploinsufficiency on cortical architecture, we analyzed the diameter of six different cortical regions along the rostral to caudal axis. Cortical thickness was significantly reduced in all analyzed areas in Tcf4-haploinsufficient (Tcf4ex4 lacZ/WT ) mice compared to their wildtype littermates (WT) (Fig. 8I–P). Moreover, the dentate gyrus volume was significantly decreased in Tcf4ex4 lacZ/WT mice (Fig. 8F–H). In addition, Tcf4ex4 lacZ/WT mice displayed agenesis of the splenium of the corpus callosum (Fig. 8F–G’). Thus, Tcf4 haploinsufficiency in mice results in anatomical deficits that resemble anatomical anomalies observed in PTHS.
Here, we used immunohistochemical analysis to systematically investigate the expression of the intellectual disability and schizophrenia-associated Tcf4 gene in the developing and adult murine CNS. Consistent with recent experimental evidence linking TCF4 to precursor proliferation, neuronal positioning and development of excitability in the developing cortex [27–29], and the suggestion that TCF4 regulates neurite branching and synapse formation in prefrontal cortical neurons , we found TCF4 to be expressed from the early precursor stage up to the post-migratory neuronal stage in the developing cortex. We also demonstrate that TCF4 is expressed in the major germinal zones for the principal neurons of the hippocampus, for cortical interneurons, as well as in developing neurons of, for example, the cerebellum and some nuclei of the amygdaloid complex. The broad expression of TCF4 strongly suggests that TCF4 regulates early and late development of cortical and subcortical structures.
As a class I bHLH TF, the transcriptional output of TCF4 is dependent on interaction with other bHLH TFs. Through such interactions, TCF4 may act as a network hub coordinating different transcriptional modules and developmental processes. The interaction partners of TCF4 in the developing CNS have not been defined. The bHLH TFs Ascl1, Neurogenin 1 and 2, NeuroD1, and NeuroD6, have a restricted developmental expression pattern and fulfill defined stage-specific functions during hippocampal and cortical development [31–33]. It is tempting to speculate that TCF4 fulfills at least part of its functions in CNS development through interaction with these proneural class II bHLH TFs.
Recent work indicated that TCF4 activity is modulated by post-translational mechanisms such as neuronal activity-dependent phosphorylation . Interestingly, we detected TCF4 immunoreactivity in fibers of the CEA, raising the possibility that TCF4 activity may be modulated by nuclear-cytoplasmatic translocation.
PTHS is associated with deficits in various functional domains including cognition, social interaction, speech, motor skills, and vegetative function, and there is some debate to which extent the PTHS phenotype may be due to post-developmental functions of TCF4 in mature neurons . Indeed, it has been shown that TCF4 directly regulates plasticity of CA1 pyramidal neurons potentially through the control of activity-dependent DNA methylation . Strikingly, we found that TCF4 is highly expressed not only in the hippocampus but also in a number of cortical and subcortical structures during adulthood, raising the possibility that TCF4 function in mature neurons is critical for plasticity in many neural circuits.
TCF4 haploinsufficiency causes PTHS, while SNPs in regulatory regions, which may cause increased TCF4 expression , are associated with an elevated risk for schizophrenia. Moreover, behavioral deficits have been reported not only for Tcf4-haploinsufficient mice but also for transgenic mice with mild TCF4 over-expression [37, 38]. These observations have led to the suggestion that TCF4 levels have to be kept in a tight range to allow for neuronal development and function. Intriguingly, we observed cell type- and region-dependent differences in TCF4 expression, raising the questions whether distinct cell populations may require different TCF4 dosages and whether there is cell type-/region-specific vulnerability to pathologically altered TCF4 expression levels.
A recent study reported similarities in the expression of TCF4 mRNA in the developing prefrontal cortex of rats and humans . Here, we show that the expression pattern of TCF4 between mice and humans is highly similar across the developing and adult brain and provide evidence that Tcf4 haploinsufficiency in mice reproduces structural anomalies that are observed in PTHS. These observations strongly suggest that the regulation and function of TCF4 is conserved between species and further validate Tcf4-haploinsufficient mice as a preclinical model for PTHS.
Our preliminary analysis indicated an irregular distribution of TCF4-expressing cells in the cortex and hippocampus of Tcf4-haploinsufficient mice strengthening the notion that TCF4 plays a role in migration and positioning of neurons . A more detailed analysis of Tcf4-haploinsufficient mice will allow to decipher the pathophysiological mechanisms underlying PTHS on the molecular, cellular, and neural circuit level and to explore experimental strategies to ameliorate cognitive and behavioral deficits in this disorder [35, 41, 42].
All animal experiments were carried out in accordance with the European Communities Council Directive (86/609/EEC) and approved by the government of Middle-Franconia, Germany. C57Bl/6NRj mice were obtained from Janvier Labs (Le Genest-Saint-Isle, France) and group-housed under a 12 h light/dark cycle with ad libitum access to food and water. Tcf4ex4WT/lacZ mice were obtained from the Wellcome Trust Sanger Institute (Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute; MGI ID: 4432303). For generation of the knockout allele, the L1L2_gt1 cassette was inserted at position 69461371 of Chromosome 18 upstream of the in silico-determined critical exon four. The L1L2_gt1 cassette harbors an FRT flanked lacZ/neomycin sequence followed by a loxP site resulting in a “knockout first” allele. An additional loxP site is inserted downstream of the targeted exon four at position 69462143 . For embryonic studies, mice were bred in the afternoon and vaginal post-coitum protein plug check (“Plug check”) was performed the next morning. This time point was defined as E0.5.
Tissue preparation and dissection
Timed pregnant mice were killed by cervical dislocation. E11.5 and E13.5 embryos were fixed overnight in 4% PFA. For the E15.5 time point, heads were fixed overnight in 4% PFA. For the E18.5 and P0 time points, brains were dissected and fixed overnight in 4% PFA. Tails were used for genotyping. After fixation tissue was washed repeatedly with 1× PBS and transferred to 30% sucrose in PBS overnight for dehydration. Embryonic tissues were embedded in freezing media (Jung, Nussloch) and stored at − 80 °C. Adult mice were killed using CO2 and transcardially perfused with PBS for 5 min (20 ml/min) followed by fixation with 4% paraformaldehyde (PFA) in PBS, pH 7.4, for 5 min. The brains were post-fixed overnight in 4% PFA at 4 °C followed by dehydration at 4 °C in 30% sucrose in TBS.
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Adult brains were coronally cut at 40 μm thickness using a sliding microtome. Immunofluorescent stainings were performed on free-floating slices. Slices were washed three times with TBS, blocked in TBS containing 0.25% Triton X-100 and 3% normal donkey serum (TBS++), and incubated with primary antibodies in TBS++ for 72 h at 4 °C. Tissue was washed with TBS at room temperature, blocked with TBS++ for 30 min at room temperature, and subsequently incubated with the secondary antibodies diluted in TBS++ overnight at 4 °C or for 2 h at RT. After washing in PBS, nuclei were stained with DAPI and sections were mounted on coverslips with Aqua poly mount (Polysciences).
Validation of TCF4 antibody
The TCF4 knockdown construct was generated cloning the TCF4 forward and reverse oligonucleotides TCF4 forward: 5′-CCGGGCTGAGTGATTTACTGGATTTCTCGAGAAATCCAGTAAATCACTCAGCTTTTT-3′; and TCF4 reverse: 5′-CGAAAAAGCTGAGTGATTTACTGGATTTCTCGAGAAATCCAGTAAATCACTCAGC-3′.
CCGG into the PWXG1 expression vector (gift from X. Zhao, University of Wisconsin, Madison). The TCF4 expression construct was generated by cloning the human TCF4 coding sequence fused with a tandem Strep II tag and a FLAG tag on the C-terminus into the CAG-IRES-GFP expression vector [43, 44]. The resulting CAG-TCF4-IRES-GFP was either transfected alone or together with the TCF4 knockdown construct in HEK 293T cells (ATCC, Wesel, Germany; CRL-3216), and the proteins were extracted 2 days post-transfection. Protein extract obtained from HEK 293T cells transfected with a GFP control construct (CAG-GFP-IRES-GFP) served as a control. Cells were lysed in RIPA buffer (50 mM Tris pH 8, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1%SDS, 0.5% S-DOC,1x protease inhibitor cocktail-EDTAfree-Roche). The extracts were separated in a 10% SDS-PAGE gel. Gels underwent wet transfer onto a nitrocellulose membrane. Membranes were blocked in 5% w/v skim milk (Sigma Aldrich) in TBS with 0.1% Tween 20 (TBS-T). Incubation with primary antibodies diluted in blocking solution was performed overnight at 4 °C and was followed by washing with TBS-T. Secondary antibodies were diluted in blocking solutions and incubated with the membranes for at least 1 h at room temperature followed by washing with TBS-T. Membranes were treated with Clarity Western Enhanced Chemiluminescence (ECL) Substrate (Bio-Rad) and visualized with ChemiDoc XRS+ System (Bio-Rad). Images were processed using ImageLab 5.2.1 Setup (Bio-Rad).
For embryonic sections, fluorescence signal was detected with an AF6000 Modular Systems Leica fluorescent microscope and documented with a SPOT-CCD camera and the Leica software LAS AF (Version 22.214.171.12466; Leica Microsystems, Wetzlar Germany). For comparison of expression levels, settings were kept constant. For expression and co-localization analysis in the adult murine brain, fluorescence signal was detected using a Zeiss LSM 780 confocal microscope with four lasers (405, 488, 550, and 633 nm) and × 20, × 40, × 63 objective lens. Images were processed using ImageJ.
Analysis of Tcf4 expression levels
For evaluation of expression levels in the murine brain, images were taken with fixed settings. Expression was categorized into four categories, i.e., high, moderate, low, and not detected, based on intensity of TCF4-immunoreactivity. For comparison of neurodevelopmental TCF4 expression between species, mRNA expression data from selected brain areas of non-human primates and humans were extracted from the NIH Blueprint Non-Human Primate (NHP) Atlas (http://www.blueprintnhpatlas.org) and the BrainSpan Atlas (BrainSpan: Atlas of the Developing Human Brain [Internet]. Funded by ARRA Awards 1RC2MH089921-01, 1RC2MH090047-01, and 1RC2MH089929-01. © 2011. Available from: http://www.brainspan.org/lcm/search?search_type=user_selections), respectively. The NIH Blueprint Non-Human Primate (NHP) Atlas provides microarray-based mRNA expression data of non-human primate neural development from E40–0 months. Microarray data of TCF4 expression in select brain regions was extracted and arranged by ascending age for documentation. The BrainSpan: Atlas of the Developing Human Brain provides microarray based mRNA expression data of human neural development from post conception week 15–21. Microarray data of TCF4 expression in select brain regions was extracted and arranged by ascending age for documentation. For comparison of TCF4 expression between human and mice during adulthood, TCF4 mRNA expression data in the adult human brain was extracted from the Allen Human Brain Atlas . Heatmaps for mRNA expression were manually compared to the immunoreactivity score.
Cortical thickness measurements
The diameter of the cortex was determined in six areas of the cortex, corresponding to the anatomical divisions documented in the Allen Mouse Brain Atlas  Posterior parietal association area (PTLp), Somatosensory area (SSp), rostral and caudal primary somatosensory area-barrel field (SSp-bfd), rostral and caudal primary somatosensory area-trunk (SSp-tr). Measurements were performed using ImageJ (version 2.0.0). Because of the variability of the pial surface, we determined the cortical thickness, as the diameter between layer II/III and the white matter. Five measurements were taken per area in each animal (n = 3 for TCF4ex4 WT/WT ; n = 6 for TCF4ex4 LacZ/WT ) and averaged prior to statistical analysis. Statistical significance was determined using the Mann-Whitney U test,*p < 0.05.
Volume measurement of the dentate gyrus
DG volumes were calculated after measuring the DG area of six consecutive 40-μm thick coronal mouse brain slices spanning a distance of 240 μm from the rostral to caudal hippocampus. Measurements were performed using ImageJ (version 2.0.0) , n = 3 for TCF4ex4WT/WT; n = 6 for TCF4ex4LacZ/WT. Statistical significance was determined using the Mann-Whitney U test,*p < 0.05.
cMRIs were obtained from two female individuals with confirmed PTHS. Individual 1 is a 5-year-old girl carrying the de novo mutation c.1880-11A>G in TCF4 which was confirmed to lead to aberrant splicing and is therefore predicted to result in frameshifting and truncation of the protein (p.(Glu623Valfs*87)). The girl has severe ID with a walking age of 4 years 9 months and lack of speech. She has PTHS typical facial features, behavioral anomalies, sleeping difficulties, constipation, and myopia. Her head circumference is on the 10th centile, and no seizures or breathing anomalies occurred so far. Individual 2 is a 7.5-year-old girl carrying the splice site mutation c.655G>A, predicted to result in frameshifting and truncation of the protein (p.(Asp219Glyfs*37)). She could walk with 2 years 9 months and speaks single words and 2–3-word sentences. She has severe constipation, muscular hypotonia, recurrent infections, and typical facial features. Her head circumference in on the 10th centile, and no seizures or breathing anomalies occurred so far. The individuals had been recruited for a study to unravel the genetic causes for developmental disorders, which was approved by the ethics committee of the Medical Faculty of the Friedrich-Alexander-Universität Erlangen-Nürnberg. All participating families had given their informed consent.
This work was supported by grants from the German Research Foundation (LI 858/9-1 to D.C.L, INST 410/45-1 FUGG), the Bavarian Research Network “ForIPS” (D.C.L.), the BMBF Network “Cell Based Regenerative Medicine” (C.J.G. and D.C.L.), iMed—the Helmholtz Initiative on Personalized Medicine (C.J.G.), the University Hospital Erlangen (IZKF grants E12, E16, and E21 to D.C.L., E26 to C.Z.). B.M.H. and E.-A. B. are doctoral fellows of the IZKF (Interdisziplinäres Zentrum für Klinische Forschung) of the University Clinic Erlangen (Project IZKF E12 and E16). M.-T.W. is a graduate student of the research training group 2162 “Neurodevelopment and Vulnerability of the Central Nervous System” of the Deutsche Forschungsgemeinschaft (DFG GRK2162/1). C.Z. and D.C.L. are investigators of the research training group 2162 “Neurodevelopment and Vulnerability of the Central Nervous System” of the Deutsche Forschungsgemeinschaft (DFG GRK2162/1). M.J. is supported by a M.D. doctoral fellowship of the Interdisciplinary Center for Clinical Research (IZKF) Erlangen. This work was performed in (partial) fulfillment of the requirements for obtaining the degree “Dr. med.”.
MJ carried out the immunohistochemical experiments, bioinformatic comparison, formal analysis and wrote the original draft of the manuscript. BMH conceptualized the experiments, performed immunohistochemical stainings, performed formal-analysis, wrote and edited the final manuscript. TT performed immunohistochemical stainings on adult mouse brain and bioinformatical comparison. M-TW performed immunohistochemical experiments on embryonic mouse brains, E-AB performed Western Blot experiments and analysis. V-CS performed Western Blot experiments. CZ recruited and examined patients, and participated in manuscript editing. AD provided MRI image resources and helped with analysis. CJG provided resources and assisted in funding acquisition. DCL conceptualized and supervised the project, performed formal analysis, provided resources and funding, wrote and edited the final manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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