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Fig. 1 | Molecular Autism

Fig. 1

From: Analysis of the expression pattern of the schizophrenia-risk and intellectual disability gene TCF4 in the developing and adult brain suggests a role in development and plasticity of cortical and hippocampal neurons

Fig. 1

TCF4 expression at different developmental stages and validation of antibody specificity. a Western blot analysis of HEK 293T transfected with either CAG-GFP-IRES-GFP (Control), CAG-TCF4-IRES-GFP/ CAG-shTCF4-IRES-GFP (TCF4 KD), or CAG-TCF4-IRES-GFP (TCF4). Detection using the anti-TCF4 antibody revealed a clear band in the TCF4 transfected cells, which was reduced in size in the TCF4 KD (left panel). Detection using an anti-FLAG antibody resulted in bands equal in height compared to the TCF4 staining and also showed reduction upon TCF4 KD (right panel). GAPDH was used as the loading control. b, c Immunostaining using the anti-TCF4 antibody of brain tissue of TCF4 wildtype (WT) and constitutive TCF4-KO mice. d–h Left-hand side of each image, TCF4 immunostaining. Right-hand side, heatmap converted image. Relative immunoreactivity scale is shown in the upper right corner: red and blue represent high and low immunoreactivity, respectively. Abbreviations: AMY, amygdala; cn, cortical neuroepithelium; CP, caudoputamen; Cx, cortex; CxP, cortical plate; DG, dentate gyrus; H, hippocampal formation; hn, hippocampal neuroepithelium; LGE, lateral ganglionic eminence; MGE, medial ganglionic eminence; pvh, paraventricular hypothalamus

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Molecular Autism

ISSN: 2040-2392