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Impaired synaptic function and hyperexcitability of the pyramidal neurons in the prefrontal cortex of autism-associated Shank3 mutant dogs

Molecular Autism volume 15, Article number: 9 (2024) Cite this article

Abstract

Background

SHANK3 gene is a highly replicated causative gene for autism spectrum disorder and has been well characterized in multiple Shank3 mutant rodent models. When compared to rodents, domestic dogs are excellent animal models in which to study social cognition as they closely interact with humans and exhibit similar social behaviors. Using CRISPR/Cas9 editing, we recently generated a dog model carrying Shank3 mutations, which displayed a spectrum of autism-like behaviors, such as social impairment and heightened anxiety. However, the neural mechanism underlying these abnormal behaviors remains to be identified.

Methods

We used Shank3 mutant dog models to examine possible relationships between Shank3 mutations and neuronal dysfunction. We studied electrophysiological properties and the synaptic transmission of pyramidal neurons from acute brain slices of the prefrontal cortex (PFC). We also examined dendrite elaboration and dendritic spine morphology in the PFC using biocytin staining and Golgi staining. We analyzed the postsynaptic density using electron microscopy.

Results

We established a protocol for the electrophysiological recording of canine brain slices and revealed that excitatory synaptic transmission onto PFC layer 2/3 pyramidal neurons in Shank3 heterozygote dogs was impaired, and this was accompanied by reduced dendrite complexity and spine density when compared to wild-type dogs. Postsynaptic density structures were also impaired in Shank3 mutants; however, pyramidal neurons exhibited hyperexcitability.

Limitations

Causal links between impaired PFC pyramidal neuron function and behavioral alterations remain unclear. Further experiments such as manipulating PFC neuronal activity or restoring synaptic transmission in Shank3 mutant dogs are required to assess PFC roles in altered social behaviors.

Conclusions

Our study demonstrated the feasibility of using canine brain slices as a model system to study neuronal circuitry and disease. Shank3 haploinsufficiency causes morphological and functional abnormalities in PFC pyramidal neurons, supporting the notion that Shank3 mutant dogs are new and valid animal models for autism research.

Background

SH3 and multiple ankyrin repeat domain (SHANK) family genes encode the scaffolding proteins SHANK1–3 at postsynaptic densities (PSD) in excitatory synapses, with SHANK gene mutations associated with autism spectrum disorder (ASD) [1]. Shank3 deficiency has been extensively studied in animal models, especially in mice, but also in rats and nonhuman primates [2]. Previous rodent studies have shown that Shank3 deletion caused abnormal behaviors relevant to ASD, including social interaction deficits and repetitive behaviors [3,4,5], which provide evidence supporting the face validity of Shank3 deficiency mouse as an autism model. Importantly, altered synaptic development, impaired synaptic function, and reduced dendritic spines at specific brain regions, due to Shank3 loss, are hypothesized as the neural substrates underlying these behavioral impairments [6,7,8]. However, the translation of Shank3 mutant rodent model outcomes to ASD patients is somewhat limited as brain structure and behavior differences exist between humans and rodents. Therefore, new animal models are required to further study autism mechanisms and treatments.

Growing evidence now suggests that the domestic dog can be used as an effective animal model to investigate human social behaviors and mental disorders as they have developed sophisticated cross-species emotional and social processing abilities during their long coevolutionary history with humans [9,10,11]. Additionally, dogs harbor brain sulcus and gyrus structures that resemble those in humans [12]. More importantly, dogs are more readily available than monkeys as they reproduce more quickly (1 versus 5 years to adulthood) and are more prolific (one versus multiple offspring per birth). We recently reported that Shank3 mutant dogs, generated by CRISPR/Cas9 editing, exhibited ASD-like behaviors, such as social withdrawal, elevated anxiety, and hypersensitive to deviant tones [13, 14], indicating that these dogs were effective ASD animal models. However, defining neuronal activity and synaptic function in such animals is required to understand the causal relationships between neuronal dysfunction and abnormal social behaviors caused by Shank3 mutations.

The prefrontal cortex (PFC) has essential roles in cognitive control, coordinating memory, and executive activity [15]. Previous patient and animal model studies implicated PFC as an important structure in social functions [2, 16, 17] as patients with ASD have structural and functional abnormalities in the PFC [18, 19]. The chemogenetic activation of pyramidal neurons in the PFC of Shank3 mutant mice elevated glutamatergic synaptic function in the PFC and restored social preference behaviors [20]. Given the crucial role of the PFC in regulating such social behaviors, we sought to characterize possible defects in PFC neuronal structure and function in Shank3 mutant dogs.

Electrophysiological recording in acute brain slices is a unique technique for studying neuronal activity and synaptic transmission due to its millisecond resolution and nanomolar sensitivity [21, 22]. Because neural network connections are preserved and structural alterations are minimal when compared to organotypic cultures for a few days, studies using acute brain slices from mammalian brains have been widely conducted [21]. Here, we first established physiological recording protocols in fresh brain slices from dogs to investigate the electrophysiological characteristics of single PFC neurons. We then examined dendritic and synaptic alterations in pyramidal neurons caused by Shank3 mutations. We observed that Shank3 loss led to hyperexcitable neuronal activity and a deficiency in glutamatergic synaptic transmission in the PFC of Shank3 mutant dogs. To our knowledge, this is the first report investigating individual neuronal properties in an autism-associated dog model. Our findings provided a possible neurobiological explanation for abnormal social behaviors in Shank3 mutant dogs.

Materials and methods

Animals

Six Beagle dogs aged ranging from 3 to 4 months were used for all experiments in this study (Additional file 1: Table S1). Among them, three male wild-type Beagle dogs were from Sinogene Ltd (Beijing, China). Three Shank3 mutant dogs (one female and two male) were heterozygous for a 496 bp deletion in exon 21 of the Shank3 gene (-496 bp/ + ; + after the slash denotes a wild-type copy of the gene); this mutation generated a frameshift and a truncated protein disrupting the proline-rich domain of Shank3 [12]. The mutant dogs showed a reduced Shank3 protein level in the brain and displayed autism-like social deficits, including social withdrawal, elevated anxiety, and reduced social interactions with humans [13]. Each dog was housed in a separate cage (1 × 1 × 1 m3) and maintained on a 12 h/12 h dark–light cycle (light on: 07:00–19:00), with a humidity of 40–60% and a temperature of 22–24 °C. The dogs were fed with regular canine food (Keao Xieli Feed Co.Ltd, Beijing, China) twice daily at 08:00–10:00 and 15:30–17:00. All animal experiments and protocols were approved in advance by The Animal Care and Use Committee of the Institute of Genetics and Developmental Biology (AP2023024).

Surgery and brain slice preparation

The dogs were anesthetized by simultaneous administration of dexmedetomidine hydrochloride (25 μg/kg, intramuscular), Zoletil 50 (5 mg/kg, intramuscular), and propofol (5 mg/kg, intravenous). After deep anesthesia, the dogs were sacrificed and transcardially perfused with ice-cold 0.9% saline (Kexing, China). The whole brain was removed, placed in a large beaker and kept submerged in cold (4 °C) cutting solution containing (in mM): 110 C5H14NClO, 2.5 KCl, 25 NaHCO3, 1.3 NaH2PO4, 7 MgCl2, 0.5 CaCl2, and 25 glucose (adjusted to pH 7.3–7.4, oxygenated with 95% O2 and 5% CO2). The PFC was sampled between the anterior and middle third of the medial frontal gyrus according to The Beagle Brain in Stereotaxic Coordinates [23]. A small block of tissue containing the PFC was excised and immediately transported to the laboratory. Coronal brain slices (300 μm thickness) were cut using a vibratome (Leica VT1200s, Wetzlar, Germany). The PFC slices were incubated for 30 min at 37 °C and then maintained at room temperature (22–25 °C) in the artificial cerebrospinal fluid (aCSF) containing (in mM): 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.3 NaH2PO4, 1.3 MgCl2, 2.5 CaCl2, and 25 glucose (adjusted to pH 7.3–7.4, oxygenated with 95% O2 and 5% CO2) for physiological recording.

Electrophysiological recording

The brain slice was transferred to a recording chamber and constantly perfused with oxygenated aCSF at 25 °C at a rate of 1.5–2.0 ml/min. Cells were visualized using IR-DIC optics on an inverted Olympus BX51WI microscope. Layer 2/3 pyramidal neurons were identified by their morphology. Whole-cell recordings were performed with Multiclamp 700B amplifier and Digidata Digitizer 1550B (Molecular Devices Corporation, Sunnyvale, CA, USA). The signals were acquired at 10 kHz and filtered at 2 kHz. Cells were voltage-clamped at − 70 mV with 4–6 MΩ patch pipettes. The access series resistance of the neurons used for analysis was < 25 MΩ.

For neuronal activity recordings, the pipette solution contained (in mM): 130 K-gluconate, 10 HEPES, 5 KCl, 4 MgCl2, 0.2 EGTA, 4 MgATP, 0.3 NaGTP, 10 Na2-Phosphocreatine. Biocytin (2 mg/ml, B4261, Sigma) was also added to the pipette solution to allow for post hoc identification of the location and morphology of recorded neurons. The intrinsic and active membrane properties were generally recorded in the presence of CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 10 μM), AP5 (2-Amino-5-phosphonopentanoic acid; 50 μM), and PTX (50 μM). Resting membrane potential was recorded immediately after breaking the seal. For analyzing action potential (AP) properties, the following parameters were calculated: AP threshold (the membrane potential of the first time dVm/dt reaching 2 mV/ms), AP amplitude (the subtraction between spike peak and AP threshold), half-width (the duration between membrane potential shoot over and drop below half amplitude), AP rise time (time between 10 and 90% of spike amplitude on depolarizing phase), decay time (time between 10 and 90% of spike amplitude on repolarizing phase), after hyperpolarization amplitude (membrane potential difference between AP threshold and the lowest potential in the hyperpolarization phase), and AHP latency (the time from AP threshold to the lowest potential of spike).

For synaptic transmission recordings, the pipette solution contained (in mM): 135 CsMeSO3, 10 HEPES, 1 EGTA, 4 MgATP, 0.3 NaGTP, 8 Na2-Phosphocreatine, 3.3 QX314 bromide (adjusted to pH 7.3 with CsOH). Evoked excitatory postsynaptic currents (EPSCs) and inhibitory postsynaptic currents (IPSCs) were recorded from the same neuron and evoked by a bipolar electrode (0.2 ms pulses) at the holding potential of − 70 mV and + 10 mV, respectively. EPSCs and IPSCs were quantified by the amplitude of the peak. All EPSCs used for analysis were averaged from 6 consecutive traces with a stimulus interval of 10 s. Data were analyzed using Clampfit 10 software.

For paired-pulse stimulation experiments, the stimulus intensity was set at a level that could evoke a response of 50% saturation for the individual cell measured and delivered with an inter-stimulus interval of 20, 50, 100, and 200 ms. The paired-pulse ratio (PPR) was calculated with the peak current response to the second pulse divided by that of the first response.

Biocytin staining

Biocytin filling and staining were performed as described previously [24]. To fully infuse the entire cell with the dye, electrophysiological recording lasted for more than 15 min. After electrophysiological recording, brain slices were fixed in neutrally buffered 4% Paraformaldehyde (PFA) solution at 4 °C overnight, washed three times in phosphate buffer saline (PBS) and then, stained with streptavidin-Cy3 (1:100 of 1 mg/ml, S6402, Sigma) for 3 h at room temperature and mounted on glass coverslips for visualization of the biocytin-filled neurons.

Analysis of dendrites and dendritic spines

Image acquisition was carried out using a Leica SP8 confocal microscope and Leica Application Suite X software (ver.1.4.5, Leica, Germany). Images were acquired at a resolution of 1024 or 2048 pixels in the X–Y dimension. The Z dimensions were variable. Most of the neurons were photographed using a 10 × objective lens for the analysis of dendritic branches. The Z-dimensional increment was 2 µm for Z-stack images. A 100 × objective lens was used for dendritic spine imaging. For the spine Z-stack images, the Z-dimensional increment ranged between 0.1 and 0.3 µm. The stack images were analyzed using Imaris software (version 9.5.1, Bitplane, Switzerland). 3D reconstruction of a single neuron was performed to remove the interference of surrounding neuronal dendrites and the total length of dendrites was automatically calculated. For the Sholl analysis, the spheres were constructed continuously from the center of the cell body with the radius increased by 10 µm. The number of intersections between each circle and dendrites was calculated for comparison.

To compare the difference in spine density between the WT and mutant dogs, spines in secondary dendrites were chosen for the analysis. The spines in the dendrites were detected by Imaris software semi-automatically. The mislabeled spines were deleted manually. Spine dimensions were defined by the length of the spine and the width of the spine neck and the spine head, which allowed to classify the spines into four types: stubby, mushroom, long thin, and filopodia. The stubby type had a length < 1 µm; the mushroom type had a length > 3 µm, and the maximum width of the head/the mean of the neck was > 2; the long thin type had a ratio of the mean width of the head/mean width of the neck ranging from 1 to 2, and the rest of the spines were filopodia. Spine measurements were performed using MATLAB-XTension Spines Classifier in Imaris.

Golgi staining

Immediately after anesthesia, the PFC tissue was removed, washed with 0.1 M PBS (4 °C), and cut into segments of 1 cm3. Following the instructions of a commercial Golgi staining kit (FD NeuroTechnologies, Inc., Columbia, MD, USA), the brain tissue was placed in a mixture of equal amounts of solution A and solution B, then stored in the dark at room temperature for 14 days. Afterward, the tissue was soaked in solution C and stored at room temperature for another 3 days. PFC tissue sections (100 μm) were made under a cryomicroscope (Leica CM1950, Germany) and placed in a mixture of solution D, solution E, and distilled water (1:1:2) for 10 min. The sections were rinsed in distilled water and then dehydrated with various gradients of ethanol (50, 75, and 95%) for 5 min each, then rinsed with xylene and sealed with resin glue. After 24 h in the dark and under ventilated condition, the slices were visualized using a bright-field microscope (Zeiss LSM980 with Airyscan2, Germany). To quantify spine density, images of at least 10–15 neurons in the PFC for each brain were taken. The number and length of dendritic spines along a 100 μm secondary dendrite were recorded using Image J software.

Transmission electron microscopy

The sample for ultrastructure analysis was prepared as described previously [25]. Briefly, fresh dog PFC slices were prepared as described above. Brain slices were then fixed with 2.5% glutaraldehyde with 0.1 M Phosphate Buffer (PB) buffer (pH 7.4) for at least 30 min at room temperature. Layer 2/3 PFC was dissected out from the brain slices under a stereoscope. Blocks were then post-fixed for 2 h with 1% osmium tetraoxide in PB for 2 h at 4 °C, dehydrated through a graded ethanol series (30, 50, 70, 80, 90, 100%, 100%, 7 min each) into pure acetone (2 × 10 min). Samples were infiltrated in a graded mixture (3:1, 1:1, 1:3) of acetone and SPI-Pon 812 resin (16.2 g SPI-Pon 812 epoxy resin monomer, 10 g dodecenyl succinic anhydride, and 8.9 g nadic methyl anhydride), then changed to pure resin. Finally, tissues were embedded in pure resin with 1.5% N, N-dimethylbenzylamine and polymerized for 12 h at 45 °C, and 48 h at 60 °C. The ultrathin sections (70 nm thickness) were sectioned with a microtome (Leica EM UC6), and collected on single-slot formvar-coated copper grids. The sections were then doubly stained with uranyl acetate and lead citrate. Images were acquired at 30,000 × magnification using a transmission electron microscope (FEI Tecnai Spirit120kV). Analysis of secretory vesicles and postsynaptic density measurements was performed using Image J by an observer who was blinded to the genotype of samples. The active zone was defined by apposition to the PSD, and vesicles in the active zone were defined as those touching the presynaptic plasma membrane or within a distance of 50 nm to the presynaptic membrane juxtaposing the PSD [26, 27]. Since single sections were used for the ultrastructural analysis, potential errors cannot be excluded.

Statistical analysis

All collected data except those cells with poor recording quality were used for statistics. Statistical analyses were performed using GraphPad Prism 8.2.1 (GraphPad Software, Inc.). We first analyzed the normality distribution of all datasets by the Shapiro–Wilk test, group differences of normality distribution data were assessed through the two-tailed unpaired t test, and data with non-normality distribution were analyzed by Mann–Whitney test or Kolmogorov–Smirnov test. Data with two independent variables were analyzed using two-way repeated measures ANOVA followed by Bonferroni’s post hoc test. Data are presented as the mean ± SEM for bar graphs or median and quartiles for violin plot. Statistical significance was set at p < 0.05.

Results

PFC pyramidal neuron hyperexcitability in acute brain slices from Shank3 mutants

To define the electrophysiological properties of neurons in our autism-associated dog model, we prepared acute brain slices from Shank3 heterozygous mutants (Mut) and wild-type (WT) dogs, and maintained slices under consistent perfusion in aCSF. A fresh coronal brain slice of dorsal lateral PFC (one of the most studied PFC subregions involving cognitive processes) was transferred to the recording chamber for electrophysiological recordings (Fig. 1A). In slices, pyramidal neurons in layer 2/3 of the PFC were recorded by whole-cell patch clamp. Pyramidal neurons were morphologically recognized under light microscopy and confirmed by biocytin staining injected from a recording pipette (Fig. 1B). To characterize the electrophysiological properties of pyramidal cells, we depolarized neurons with a series of gradient-increasing current injections. Pyramidal neurons showed no spontaneous firing at baseline but initiated action potentials (APs) with a current injection of ≥ 50 pA and reached a maximum firing frequency of approximately 10 Hz (Fig. 1C, D), similar to electrophysiological properties of neocortical pyramidal neurons in humans [28, 29], rodents [30], and nonhuman primates [31].

Fig. 1
figure 1

Hyperexcitability of pyramidal neurons in the PFC slices of Shank3 heterozygous mutant dogs. A Schematic illustrating the preparation of neocortex slices and electrophysiological recording. B A representative image of a recorded layer 2/3 pyramidal neuron in fresh PFC brain slices. Scale bar, 10 μm. C Representative spiking pattern of pyramidal neurons in response to − 50 pA, 0 pA, and + 200 pA current injections. D–G Compared with WT controls, PFC pyramidal neurons from Shank3 mutant dogs displayed D a left-shifted current-response curve (WT: n = 27 neurons, Shank3 mutant: n = 20 neurons; p = 0.0325), E decreased rheobase (WT: n = 21 neurons, Shank3 mutant: n = 21 neurons; p = 0.0437), and no change in F resting membrane potential (WT: n = 19 neurons, Shank3 mutant: n = 21 neurons; p = 0.9359) and G capacitance (WT: n = 22 neurons, Shank3 mutant: n = 22 neurons; p = 0.9371). Data in E–G were analyzed with a two-tailed, unpaired t test. Data in D were analyzed with two-way repeated measure ANOVA. All data were collected from 3 pairs of animals, and presented as mean ± SEM; *p < 0.05, **p < 0.01 and ***p < 0.001

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We then examined the intrinsic excitability of pyramidal cells and found that layer 2/3 pyramidal neurons in Shank3 mutant dogs were more hyperactive than those in WT dogs, with a left-shifted current-response curve (Fig. 1C, D). Consistently, rheobase currents were decreased (WT: 136.2 ± 17.13 pA, Mut: 92.38 ± 12.07 pA; p = 0.0437) (Fig. 1E), while the resting membrane potential and membrane capacitance remained normal in Shank3 mutants compared to WT controls (Fig. 1F, G), suggesting that intrinsic excitability in pyramidal neurons was increased in Shank3 mutant dogs. However, AP properties such as AP threshold, AP amplitude, AP rise time, and after hyperpolarization amplitude appeared normal but showed slightly prolonged decay times and half-widths in Shank3 mutants (Additional file 1: Fig. S1 A–H). These results showed that intrinsic excitability in PFC pyramidal cells was increased in Shank3 mutant dogs.

Impaired synaptic transmission of layer 2/3 pyramidal neurons in Shank3 mutant dogs

Previous studies have reported impaired synaptic functions which were hypothesized as mechanism underlying behavioral abnormalities in Shank3 mutant rodents [2, 7, 30, 32,33,34,35]. To determine if synaptic function was impaired in Shank3 mutant dogs, we examined evoked excitatory postsynaptic current (EPSC) amplitudes in response to different stimulus intensities (20%, 50%, and 100% saturation). We found that EPSCs in layer 2/3 pyramidal neurons in Shank3 mutants showed reduced trends when compared to WT controls. In particular, EPSCs in response to lower stimulus intensity (20% saturation) were significantly decreased in Shank3 mutants when compared to WT controls (WT: 104.6 ± 7.47 pA, Mut: 78.27 ± 9.34 pA; p = 0.0351), suggesting impaired glutamatergic synaptic transmission in pyramidal neurons (Fig. 2A, B). However, inhibitory postsynaptic currents (IPSCs) in Shank3 mutant dogs in response to various stimulus intensities were comparable to WT dogs (Fig. 2A, C).

Fig. 2
figure 2

Impaired glutamatergic synaptic transmission of layer 2/3 pyramidal neurons in Shank3 mutant dogs. A The representative EPSCs and IPSCs of PFC pyramidal neurons from WT and Shank3 mutant dogs. EPSCs and IPSCs were evoked by 20%, 50%, and 100% saturation stimulus. B Comparison of EPSCs amplitude between WT and Shank3 mutant dogs (WT: n = 8 neurons, Shank3 mutant: n = 10 neurons; p = 0.0351, 0.7186, and 0.7889 for stimulus intensities at 20%, 50%, and 100% saturation levels, respectively). C Comparison of IPSCs amplitude between WT and Shank3 mutant dogs (WT: n = 8 neurons, Shank3 mutant: n = 11 neurons; p = 0.8698, 0.6016, and 0.7939 for stimulus intensities at 20%, 50%, and 100% saturation levels, respectively). D The normalized EPSCs and IPSCs in response to paired pulses with intervals of 50 ms and 200 ms. E Paired-pulse ratio of EPSCs of WT and Shank3 mutant dogs (WT: n = 14 neurons, Shank3 mutant: n = 12 neurons; p = 0.0003, 0.0236, 0.0648, and 0.0159 for paired-pulses intervals at 20 ms, 50 ms,100 ms, and 200 ms, respectively). F Paired-pulse ratio of IPSCs of WT and Shank3 mutant dogs (WT: n = 13 neurons, Shank3 mutant: n = 13 neurons; p = 0.6568, 0.6435, 0.6527, and 0.6097 for paired-pulses intervals at 20 ms, 50 ms,100 ms, and 200 ms, respectively). G Representative spontaneous EPSCs (sEPSCs) of PFC pyramidal neurons from WT and Shank3 mutant dog. H, J Cumulative distributions of amplitude and inter-events interval of sEPSCs (WT: n = 10 neurons, Shank3 mutant: n = 15 neurons; p < 0.0001). I, K The histograms for the amplitude (I) and frequency (K) of sEPSCs. Data in B, C, E, F, I and K were analyzed with a two-tailed, unpaired t test. Data in H and J were analyzed with Kolmogorov–Smirnov test. All data were collected from 3 pairs of animals, and presented as mean ± SEM; *p < 0.05, **p < 0.01 and ***p < 0.001

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We then examined presynaptic release probability by calculating paired-pulse ratios (PPRs) of EPSCs and IPSCs. The PPR, which is the ratio of the amplitude of the second response to that of the first, has been successfully used to calculate vesicular release probability at presynapses [36]. While the PPR of IPSCs did not change, the PPR of EPSCs in Shank3 mutant dogs was lower than WT dogs, indicating an enhanced presynaptic glutamate release probability (Fig. 2D–F). This likely occurred as a side-effect of presynapses compensating for long-term postsynaptic deficiency. Furthermore, the amplitude of spontaneous EPSCs (sEPSCs) was not affected but the sEPSC frequency was decreased due to Shank3 loss (Fig. 2G–K). Taken together, these results suggested that glutamatergic synaptic transmission onto layer 2/3 pyramidal neurons in the PFC was impaired in Shank3 mutant dogs.

Reduced dendrite complexity and spine density in Shank3 mutant dogs

Neocortical pyramidal neurons are morphologically characterized by distinct basal and apical dendrites that receive distinctly organized synaptic inputs from different afferents [37]. The aforementioned synaptic deficits in this study may have been caused by changes in dendrite elaboration or spine morphology and density brought on by Shank3 mutations. To determine if neurons showed morphological changes in Shank3 mutant dogs, pyramidal neurons were filled with biocytin during electrophysiological recordings and counterstained with streptavidin-Cy3. High-resolution confocal z-stack scans were acquired (Fig. 3A), stitched with ImageJ, and digitally reconstructed using Imaris software for analyzing the dendritic spine density and morphology (Table 1).

Fig. 3
figure 3

Decreased dendrite complexity and spine density in the PFC of Shank3 mutant dogs. A Representative images (left panel) and 3D reconstruction (right panel) of biocytin-filled pyramidal neurons in the layer 2/3 PFC from Shank3 mutant and WT dogs. The neurons were filled with biocytin via patch pipette and stained with streptavidin-Cy3. Scale bars, 100 μm. B Analysis of the total dendrites length of pyramidal neurons from Shank3 mutant and WT dogs (WT: n = 9 neurons, Shank3 mutant: n = 13 neurons; p = 0.2767). C Sholl analysis of dendritic branching complexity in the PFC pyramidal neurons from WT and mutant dogs (WT: n = 9 neurons, Shank3 mutant: n = 13 neurons; p = 0.0494). D The representative images showing the dendritic spines of layer 2/3 pyramidal neurons from WT and Shank3 mutant dogs: the confocal slice (left), 3D reconstruction of spines (middle), and the merged image (right). Scale bar, 5 μm. E Averaged spine length of pyramidal neurons obtained from WT and Shank3 mutant dogs (WT: n = 9 neurons, Shank3 mutant: n = 13 neurons; p = 0.1620). F The spine density on the dendrites of pyramidal neurons obtained from WT and Shank3 mutant dogs (WT: n = 9 neurons, Shank3 mutant: n = 13 neurons; p < 0.0001). G Comparisons of densities for stubby (p = 0.0011), mushroom (p = 0.9729), and long thin spines (p = 0.0056) between WT and Shank3 mutant dogs. Data in B, E, and F were analyzed with a two-tailed, unpaired t test. Data in C and G were analyzed with Mann–Whitney test. All data were collected from 3 pairs of animals. Data points represent neurons (circle) and dogs (rhombus) for each group, and presented as mean ± SEM; *p < 0.05, **p < 0.01 and ***p < 0.001

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Table 1 Numbers of animals, neurons, dendrites, spines, and synapses examined in the morphological and EM analysis
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Labeled neurons presented with a typical pyramidal neuronal shape with distinct apical dendrites, extensive basal dendritic trees, and spines on dendrites. Total dendrite length was quantified for neurons, with no significant differences in dendritic length between Shank3 mutant and WT dogs (WT: 7,730 ± 1489 µm, Mut: 5,936 ± 856.5 μm, p = 0.2767; Fig. 3B). However, Sholl analysis at 10 µm incremental distances from the cell soma showed that the number of dendrite intersections in pyramidal neurons from Shank3 mutants was decreased when compared to WT dogs (Fig. 3C), indicating reduced dendritic complexity and consequently reduced synaptic connections with presynaptic terminals. Although averaged spine length was not significantly altered (WT: 1.57 ± 0.08 μm, Mut: 1.71 ± 0.06 μm, p = 0.1620; Fig. 3D, E), total dendritic spine density in Shank3 mutant dogs was strikingly lower than that of WT dogs (WT: 14.71 ± 0.94/10 μm, Mut: 6.82 ± 0.71/10 μm, p < 0.0001; Fig. 3D, F). Similar results were also observed in PFC pyramidal neurons labeled by Golgi staining (Additional file 1: Fig. S2 A, B). These results together showed that PFC pyramidal neurons exhibited neuronal structural deficits in Shank3 mutant dogs, consistent with our findings of impaired synaptic transmission onto pyramidal neurons (Fig. 2).

Dendritic spines are classified as thin, stubby, mushroom, and filopodia [38, 39] structures based on their length and the relative size of their head and neck. Mushroom spine density (WT: 0.34 ± 0.05/10 μm, Mut: 0.33 ± 0.05/10 μm; p = 0.9729) was not significantly altered in Shank3 mutant dogs when compared to WT dogs, but stubby spine (WT: 3.30 ± 0.63/10 μm, Mut: 1.14 ± 0.26/10 μm; p = 0.0011) and long thin spine (WT: 7.17 ± 0.75/10 μm, Mut: 4.27 ± 0.67/10 μm; p = 0.0056) densities were remarkably reduced (Fig. 3G). As few filopodia spines were available, a statistical analysis was not conducted. Notably, we used 3–4-month-old juvenile dogs for experiments because autism has a high incidence in childhood. At this age, we observed more stubby and long thin spines when compared with mushroom spines in dog PFC, consistent with the notion that stubby spines are predominant during early postnatal development in mice [40].

Excitatory synapse PSD is smaller in the PFC of Shank3 mutant dogs

To further examine neuron morphological changes in Shank3 mutant dogs, we examined excitatory synapse ultrastructure by electron microscopy (Table 1). Excitatory synapses were well-recognized by asymmetric pre- and postsynaptic structures. Each excitatory synapse was characterized by a cluster of round presynaptic vesicles and a robust PSD, which is an electron-dense thickening underneath postsynaptic membranes [41]. We observed that PSDs were shorter (WT: 517.5 ± 16.2 nm, Mut: 399.7 ± 11.6 nm; p < 0.0001) and thinner (WT: 85.0 ± 3.3 nm, Mut: 70.5 ± 2.2 nm; p = 0.0003) in Shank3 mutant dogs when compared to WT dog (Fig. 4A–C). However, Shank3 mutant dogs showed a higher vesicle density in the active zone of excitatory synapses (WT: 209.0 ± 5.2/µm2, Mut: 304.9 ± 5.3/μm2, p < 0.0001; Fig. 4D), in agreement with increased vesicle release probability (Fig. 2D, E) and were likely generated as a result of compensatory mechanisms for long-term synaptic deficits.

Fig. 4
figure 4

Smaller PSD of the excitatory synapse in Shank3 mutant dogs. A Electron microscope images of excitatory synapses in layer 2/3 of PFC. White arrows indicate postsynaptic densities (PSD). Pre, presynapse; scale bar 200 nm. B PSDs are shorter in Shank3 mutants compared with WT controls (p < 0.0001). C PSDs are thinner in Shank3 mutants compared with WT controls (p = 0.0227). D Increased density of presynaptic vesicles at the excitatory synapses in mutant dog (p < 0.0001). Data in B–D were analyzed with Mann–Whitney test. n = 228 synapses from WT dogs, and n = 213 synapses from Shank3 mutants, data are presented as median and quartiles. The data points for each dog are presented as rhombus, *p < 0.05, **p < 0.01, and ***p < 0.001

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Discussion

Shank3 mutant dogs exhibit autism-like social deficits, including social withdrawal, elevated anxiety, and greater sensitivity to deviant tones [13, 14]. In the present study, we found that the synaptic transmission of layer 2/3 pyramidal neurons in the PFC was decreased, and accompanied by reduced dendritic spine density in Shank3 mutant dogs when compared to WT dogs. Additionally, pyramidal neuron excitability was enhanced in Shank3 mutant dogs. These results suggested that PFC pyramidal neuron morphologies and functions were altered by Shank3 haploinsufficiency, which may be an underlying mechanism of abnormal behaviors observed in Shank3 mutant dogs [13, 14].

Electrophysiological recording in acute brain slices from mammalian brains is an important technique for studying neuronal activity and synaptic connections as an alternative to in vivo recordings [42, 43]. However, due to experimental animal limitations arising from ethical considerations, electrophysiological recordings, especially patch clamp, are rarely applied to large animals [28, 44, 45]. Here, for the first time, we prepared acute PFC brain slices from dogs following rodent protocols and performed whole-cell recordings on single pyramidal neurons. The majority of recorded neurons were in good condition as measured by regular spiking patterns, resting membrane potentials, and synaptic responses. The electrophysiological properties of canine PFC layer 2/3 pyramidal neurons were similar to rodents and primates [8, 28, 30, 31]. This work demonstrated the feasibility of electrophysiological recordings in brain slices to reveal physiological and pathological neuronal properties in canines.

We observed that PFC pyramidal neurons from Shank3 mutant dogs exhibited higher excitability than WT animals (Fig. 1C, D), along with reduced rheobase currents (Fig. 1E). Similar results were previously reported in striatal medium spiny neurons from Shank3 null mice carrying a deletion of protein-coding exons 4–22 [46], layer 2/3 pyramidal neurons in medial PFC from a Shank3 mouse line with deleted exons 14–16 [30], and human neurons harboring a SHANK3 (exon 13) deletion [47]. These findings, together with increased neuronal excitability in PFC layer 2/3 neurons from Shank3 mutant dogs observed in our study, suggested that increased neuronal excitability was a conserved phenotype caused by Shank3 deletions. However, how Shank3 is implicated in neuronal excitability regulation remains an enigma. Yi et al. [47] provided evidence that SHANK3 haploinsufficiency caused dysfunction of the hyperpolarization-activated cyclic nucleotide-gated channel (HCN) in cell membranes as the SHANK3 protein directly bound to HCN proteins, which in turn caused hyperexcitability in human neurons. Further studies are required to clarify the relationships between decreased synaptic transmission and increased neuronal excitability caused by Shank3 loss. Additionally, it will be important to determine if the nonsynaptic effects of increased neuronal excitability contribute to autistic behaviors.

Excitatory synaptic transmission onto PFC layer 2/3 pyramidal neurons and spine density were impaired in Shank3 mutant dogs (Figs. 2 and 3), consistent with the presence of SHANK3 in excitatory postsynaptic specializations where the protein acts as a scaffold to coordinate receptor signaling [48, 49]. Similar results were previously reported in different brain regions (PFC, hippocampus, striatum, or cerebellum) in multiple Shank3 deficient cell or animal models of different species, including humans, macaques, mice, and rats [3, 8, 50,51,52,53]. Although synaptic impairments are hallmarks of Shank3 deficiency, not all Shank3 deficient animal models showed this phenotype. For instance, when compared with controls, no changes in dendrite complexity and spine density were observed in medial PFC pyramidal neurons in homozygous and heterozygous Shank3 deficient rats [54], nor in the hippocampus of Shank3 mutant mice carrying an exon 21 deletion [55]. Whether synaptic connections are altered in Shank3 deficient animals may depend on animal age, the brain region, cell type, and the degree and dose of the Shank3 deletion.

Shank3 mutant dogs in our study represented hypomorphs where the proline-rich domain encoded by exon 21 of Shank3 was disturbed. Exon 21 is the largest coding exon of SHANK3. Rare pathogenic single nucleotide variants within exon 21 have been reported in individuals with ASD [56, 57]. Both mouse and macaque models, with various exon 21 mutations, showed impaired social interactions [55, 58]. In addition to homozygous Shank3 deficient mice with exon 21 deletion, heterozygous Shank3-deficient mice (Shank3+/ΔC) also exhibited reduced social behaviors and impaired synaptic transmission, albeit less severe than homozygous mutant phenotypes [35, 59, 60]. Our work has confirmed that Shank3 haploinsufficiency induced morphological and functional abnormalities in Shank3 mutant dogs. As ASD patients, including patients with Phelan-McDermid syndrome, typically carries heterozygous SHANK3 mutations [61, 62], Shank3 heterozygous mutant animals may better mimic the pathophysiology and behavioral abnormalities of ASD patients.

Dogs at the age of 3–4 months were analyzed in the present study. There are two reasons for this: the brain slices from the younger animals are easier for electrophysiological recordings than from adults; the other is, 3–4 months beagle dogs are in their childhood, and during this time, ASD occurs in humans. According to a recent study [63], the 3–4 months old dogs correspond to human childhood. Therefore, we believe that the dog age of 3–4 months is an appropriate time for the present study because it captures the high occurrence of ASD in children and is also when synaptic development tends to stabilize. We note, however, the brains of individuals with ASD at different age groups undergo different changes as previously reported [19, 64]. For animal welfare and practical considerations, we can only choose animals from a certain age group or developmental stage for comparative research at the moment. As a result, we are unable to determine whether our findings apply to the autistic dog models at other ages.

Limitations

We identified altered PFC pyramidal neuron morphologies and functions in Shank3 mutant dogs, but it remains unclear how PFC neuropathology is linked to behavioral alterations. Future work should address this question by manipulating neuronal activity or restoring synaptic transmission in the PFC of Shank3 mutant dogs. Because Shank3’s function in synaptic transmission regulation varies depending on the brain region and neural development stage, spatiotemporal neuropathology features and social behaviors need to be further defined.

Conclusions

Collectively, pyramidal neuron hyperexcitability, reduced dendrite elaboration and spine densities, and impaired synaptic transmission showed that Shank3 haploinsufficiency induced morphological and functional abnormalities, providing neurobiological insights underlying social behavior abnormalities. Our work also demonstrated the feasibility of using canine brain slices as a model system to study neuronal circuitry and disease, thereby shedding new light on neuronal mechanisms underlying ASD-associated behaviors.

Availability of data and materials

The datasets used and/or analyzed in the current study are available from the corresponding author upon request.

Abbreviations

ASD:

Autism spectrum disorder

PFC:

Prefrontal cortex

PSD:

Postsynaptic density

AP:

Action potential

EPSCs:

Evoked excitatory postsynaptic currents

IPSCs:

Inhibitory postsynaptic currents

PPR:

Paired-pulse ratio

PFA:

Paraformaldehyde

PBS:

Phosphate buffer saline

sEPSCs:

Spontaneous EPSCs

HCN:

Hyperpolarization-activated cyclic nucleotide-gated channel

Mut:

Mutant

WT:

Wide type

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Acknowledgements

We thank Can Peng and Xixia Li for helping with electron microscopy sample preparation and taking images at the Center for Biological Imaging (CBI), Institute of Biophysics, the Chinese Academy of Sciences.

Funding

This work was supported by STI2030-Major Projects (2021ZD0203900), the National Natural Science Foundation of China (31830036 and 31921002 to Y. Zhang, 82101568 to F. Zhu., and 32100783 to H. Zhao).

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  1. Feipeng Zhu and Qi Shi authors contributed equally.

Authors and Affiliations

  1. State Key Laboratory for Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Feipeng Zhu, Qi Shi, Yong Q. Zhang & Hui Zhao

  2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Qi Shi & Yong Q. Zhang

  3. Department of Genetics and Neuroscience, Yale University School of Medicine, New Haven, CT, 06510, USA

    Yong-hui Jiang

  4. School of Life Sciences, Hubei University, Wuhan, 430415, China

    Yong Q. Zhang

  5. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China

    Hui Zhao

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Contributions

YQZ and YHJ conceptualized the project, supervised data collection and analysis. FZ designed and performed the experiments, QS and HZ performed experiments and analyzed all data; FZ wrote the paper with input from all authors.

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Supplementary Information

Additional file 1:

 Supplementary table and figures. Table S1. List of juvenile Shank3 mutant dogs used in the present study. Figure S1. Action potential properties of PFC pyramidal neurons in Shank3 mutants and WT dogs. Figure S2. Decreased spine density in the PFC of Shank3 mutant dogs by Golgi staining.

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Zhu, F., Shi, Q., Jiang, Yh. et al. Impaired synaptic function and hyperexcitability of the pyramidal neurons in the prefrontal cortex of autism-associated Shank3 mutant dogs. Molecular Autism 15, 9 (2024). https://doi.org/10.1186/s13229-024-00587-4

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Keywords

Molecular Autism

ISSN: 2040-2392