Fig. 4

Smaller PSD of the excitatory synapse in Shank3 mutant dogs. A Electron microscope images of excitatory synapses in layer 2/3 of PFC. White arrows indicate postsynaptic densities (PSD). Pre, presynapse; scale bar 200 nm. B PSDs are shorter in Shank3 mutants compared with WT controls (p < 0.0001). C PSDs are thinner in Shank3 mutants compared with WT controls (p = 0.0227). D Increased density of presynaptic vesicles at the excitatory synapses in mutant dog (p < 0.0001). Data in B–D were analyzed with Mann–Whitney test. n = 228 synapses from WT dogs, and n = 213 synapses from Shank3 mutants, data are presented as median and quartiles. The data points for each dog are presented as rhombus, *p < 0.05, **p < 0.01, and ***p < 0.001