Fig. 4

Detection of RHOA (a, b) and of the phoshorylated form of MLC (P-MLC, c, d) by western blots in heterozygous Kctd13^+/− (a, c) and the 16p11.2 Del/+ (b, d) hippocampal lysates and their control (wt) littermate. a The quantification of the western blot (an example is shown below the graph) revealed no changes in RHOA protein levels in the Kctd13^+/− (A) or in the 16p11.2 Del/+, b mutant lines compared to their wt littermates. Fasudil treatment did not cause changes in RHOA protein levels in the two mutant lines [non-treated wt (n = 22), treated wt (n = 11), non-treated Kctd13^+/− (n = 21) and treated Kctd13^+/− (n = 7); and non-treated wt (n = 17), treated wt (n = 8), non-treated Del/+ (n = 11) and treated Del/+ (n = 11)]. However, Kctd13 deficient mice showed an increase in the levels of phosphorylated MLC protein (c) and the loss of a copy of 16p11.2 region caused an increase in the levels of phosphorylated MLC protein (d). The treatment with fasudil reversed this alteration in Kctd13^+/− and in the Del/+ mutant line. (*p < 0.05; **p < 0.01)