Fig. 3

Transwell assay of GM-CSF MΦ. A Summary of the transwell assay. B Representative images of immunostaining of MAP2 + dendrite cultured with TD- or ASD-GM-CSF MΦ indirectly at DIV56. All neurons were induced from 201B7 hiPSC line, and co-cultured with macrophages of TD3 or ASD1 were used in these images. Scale bar: 100 μm. C–G Results of (C) total length of MAP2 + dendrite, D average length of MAP2 + dendrite, E max length of MAP2 + dendrite, F MAP2 + dendrite count, and G branch point count of MAP2 + dendrite. Similar dendritic shortening to the results of direct co-culture was observed in transwell assay. C Unpaired t test, t = 3.592, p = 0.0006. D Welch’s t test, t = 2.010, p = 0.0484. E Unpaired t test, t = 2.956, p = 0.0041. F Unpaired t test, t = 1.757, p = 0.0830. G Unpaired t test, t = 1.960, p = 0.0536. n(TD-GM-CSF MΦ transwell) = 39 fields of 19 independent dishes from five times differentiations of two control healthy hiPSC lines each, and n(ASD-GM-CSF MΦ transwell) = 40 fields of 20 independent dishes from five times differentiations of two control healthy hiPSC lines each. *p < 0.05, **p < 0.01, ***p < 0.001