Fig. 1

Tau and Mapt mRNA levels are increased in the cortex of Fmr1 KO mice. A Tau protein in the cortex and hippocampus of WT and Fmr1 KO mice (FVB background, 1.5-month-old) was detected by western blotting; and protein levels were quantified by densitometry for comparison after normalizing to those of GAPDH. WT: n = 6; Fmr1 KO: n = 6. B Mapt mRNA levels in the cortex and hippocampus of WT and Fmr1 KO mice (FVB background, 1.5-month-old) were detected by qRT-PCR and compared after normalizing to those of β-actin. WT: n = 4; Fmr1 KO: n = 4. C FMRP protein was detected by western blotting in the cortex and hippocampus of WT and Mapt KO mice (C57BL/6/SV129 background, 4-month-old); and protein levels of three major FMRP isoforms (1, 2, and 3) were individually quantified by densitometry for comparison after normalizing to those of β-actin. WT: n = 3; Mapt KO: n = 3. D Fmr1 mRNA levels in the cortex and hippocampus of WT and Mapt KO mice (C57BL/6/SV129 background, 4-month-old) were detected by qRT-PCR and compared after normalizing to those of β-actin. WT: n = 3; Mapt KO: n = 3. Unpaired t test. ns: not significant; *p < 0.05, **p < 0.01