Fig. 6

Characterization of basal behaviour in C58 mice. A Basal body temperature at 8am, as measured by subcutaneously implanted transponder (°C). B Spontaneous behaviour assessed by the amount of burrowed food pellets at 2 h, and overnight, and by marble burying for 20 min. C Description of the repetitive behaviours found in C58 strain: backflips and upright scrabbles for 30 min. D Analyses of the general activity using the open-field test, showing the mean speed (m/s), number of rotations, time rearing (s) and travelled distance (m) for 10 min. E Social behaviour characterization using the frequency of sniffs and the time of sniffing of a mouse in comparison with an inanimate object (ratio mouse:object) for 10 min. F Experimental scheme for assessment of the effect of WBH on behavioural parameters in C58 mice. G Impact of WBH on 5 behavioural indices, measured at 5 h after the beginning of WBH. Each animal is represented by a single data point and mean ± SEM are also indicated (n = 24 for each strain in (A–E) and 11–12 in (G). Data have been analysed by parametric or nonparametric statistics as appropriate to the data distribution: unpaired two-tailed t-test or Mann–Whitney, *, ** and *** denote p < 0.05, p < 0.01 and p < 0.001 in pairwise comparisons