Fig. 7

Foxp1 is required for the dendrite development of layer IV neurons. A Representative Golgi-Cox staining in Foxp1-cKO and WT control mice at 6 weeks of age. Scale bar, 1 mm. B Photomicrographs of spiny stellate neurons (arrows) stained with Golgi-Cox in the barrel cortex. Scale bar, 50 μm. C Reconstruction of individual layer IV stellate neurons from WT and the KO brains. Scale bar, 50 μm. D Histogram shows the proportions of cells with asymmetric dendrite orientation. Symbols (Blue circles: WT; Red circles: Foxp1-cKO) represent the single data points for each animal (13–25 cells per animal, n = 3 animals per genotype). ***p < 0.001. E The number of dendritic intersections with Sholl circles at increasing distances from the center of the cell soma. The KO mice had significantly fewer intersections with circles 40 to 120 μm away from the soma. Circles represent the average of all analyzed cells for each animal (13–25 cells per animal), n = 3 brains per genotype. *p < 0.05, **p < 0.01, ***p < 0.001. F–H Quantification of the total length of dendrites, span area, and tree length of layer IV neurons in S1. Circles (Blue: WT; Red: Foxp1-cKO) represent the average of all analyzed cells for each animal (13–25 cells per animal), n = 3 brains per genotype. **p < 0.01