Fig. 5

Foxp1 regulates barrel formation in S1. A and B Brain coronal sections from P7 WT and Foxp1-cKO mice subjected to VGluT2 (A) or 5-HT (B) immunostaining. Arrow delineates the barrel units in layer IV of S1. The barrel patches from TCA were almost invisible in Foxp1-cKO mice. Scale bar, 200 μm in (A) and (B). C and D Quantifications of VGluT2 (A) and 5-HT (B) fluorescence intensity of layer IV. Circles represent the average fluorescence intensity for each animal (3 animals per genotype; 3 sections per animal). ***p < 0.001. E A representative image showing 5-HT staining on flattened cortices from P7 WT and Foxp1-cKO mice. Scale bar, 200 μm