Fig. 4

C-Fos is not induced in layer IV but increased in BLA of Foxp1-cKO mice by whisker stimulation. A–B c-Fos immunolabeling in the barrel cortex (A), hippocampus (A), and BLA (B) of WT and cKO mice 2 h following repeated whisker stimulation. White dot lines indicate the border between the cortex and hippocampus. Scale bars, 500 μm in (A), 200 μm in (B). C–E Quantification of c-Fos positive cells in cortical layers of WT and Foxp1-cKO mice. Circles (Blue: WT; Red: Foxp1-cKO) represent the average cell number for each animal (3 animals per genotype; 3 sections per animal). Ns, no significant difference; **p < 0.01. F–G Statistics of c-Fos positive cells in the hippocampus (F) and BLA (G). Circles represent the c-Fos⁺ cell density for each animal (3 animals per genotype; 3 sections per animal). Ns, no significant difference; ***p < 0.001