Fig. 1

Cortical knockout of Foxp1 impairs development of cerebral cortex. A Immunostaining of FOXP1 (green) on the coronal sections of P7 mouse brains. Nuclei were stained with DAPI (blue). Foxp1 was absent from the cortex and hippocampus of Foxp1-cKO mouse brains. B Foxp1 was expressed in the striatum of both WT and KO mouse brains. II-VI, cortical layers; WM, white matter; Hp, hippocampus. C Representative image of whole brains from P35 WT and Foxp1-cKO mice. D Histogram of brain weights. Circles (Blue: WT; Red: Foxp1-cKO) represent the single data points for each brain (n = 6–7 animals). ***p < 0.001. E Coronal sections of cerebral cortex from P35 WT and Foxp1-cKO mice. Scale bar, 50 μm in (A), (B), and (E). F Histogram of the cortical thickness at P35. Circles represent the average thickness for each animal, n = 4 brains per genotype. **p < 0.01