Fig. 3
From: Shank2 identifies a subset of glycinergic neurons involved in altered nociception in an autism model
Shank2 expression in post-mortem human spinal cord tissue shows similar distribution compared to mice. Identification of shank2 expression in post-mortem human spinal cord a Clarity and IF staining of Human spinal cord samples reveals a subset of Shank2high expressing cells in laminae III, IV and IV (white arrows). N = 5, scale bar overview: 500 µm; scale bar insert: 20 µm. b and c IF staining and high magnification imaging of Human spinal cord samples revealed expression of Shank2 across the cell body and dendrites, Laminae III and IV have neurons with a higher density of Shank2 expression on both cell body and dendrites like neurons in mouse spinal cord; (N = 3; n = Laminae I: 30; Laminae II: 33; Laminae III: 35; Laminae: 37 neurons), scale bar: 10 µm. Data shown as scatterplots