Fig. 5

Mdiv-1 treatment. A Rescue experiment with Drp1 inhibitor Mdiv-1. Analysis of mitochondrial fragmentation and distribution in Mito-dsRed infected cortical neurons (Setd5^+/+ and Setd5^+/−, Setd5^+/− mdvi-1 treated) live imaging (scale bar = 20-micron, magnification, scale bar = 5 micron). Statistics (fragmentation, one-way ANOVA with Tuckey test for multiple comparisons; Setd5^+/+ vs. Setd5^+/−, P < 0.0001; Setd5^+/+ vs. Setd5^+/− Mdvi-1, P = 0.0106; Setd5^+/− vs. Setd5^+/− mdvi-1, P < 0.0001. MITO area in soma, one-way ANOVA with Tuckey test for multiple comparisons; Setd5^+/+ vs. Setd5^+/−, P < 0.0001; Setd5^+/+ vs. Setd5^+/− mdvi-1, P = 0.0513; Setd5^+/− vs. Setd5^+/− mdvi-1, P < 0.0001. MITO area in neurites, statistics, two-way ANOVA (row factor, P < 0.0001, column factor, P = 0.0008, subject, P = 0.51), multiple comparison Tuckey test (Setd5^+/+ vs. Setd5^+/−, 10 um, P = 0.6279, 20 um, P = 0.6306, 30 um, P = 0.2004, 40 um, P < 0.0001, 50 um, P < 0.0001; Setd5^+/+ vs. Setd5^+/− mdivi-1, 10 um, P = 0.6698, 20 um, P = 0.9999, 30 um, P = 0.9465, 40 um, P = 0.8264, 50 um, P = 0.9159; Setd5^+/− vs. Setd5^+/− mdivi-1, 10 um, P = 0.9547, 20 um, P = 0.7055, 30 um, P = 0.1319, 40 um, P = 0.3155, 50 um, P = 0.0261). All Data are presented as mean values+/− SEM