Fig. 4

Mitochondrial structure and function in Setd5+/− mice. A In vivo ATP production from isolated mitochondria from the cortex. PM (pyruvate/malate substrate), GM (glutamate/malate). Statistics, PM, Tuckey test, (Setd5^+/+ vs. Setd5^+/−, P = 0.0791). GM, Tuckey test, (Setd5^+/+ vs. Setd5^+/−, P = 0.05). B Ultrastructural analysis by transmission electronic microscopy (TEM) of mitochondria in Setd5^+/+ and Setd5^+/− mouse cortex (scale bar = 500 nm). Quantification of the mitochondria major axis length is shown on the right. Statistics, Mann − Whitney test (Setd5^+/+ vs. Setd5^+/−, P < 0.0001). C Cortical Neurons mitochondrial fragmentation and distribution analysis. Mito-dsRed infected cortical neurons (Setd5^+/+ and Setd5^+/−) live imaging (scale bar = 20 micron, magnification, scale bar = 5 micron). MITO fragmentation, statistics, T-test (Setd5^+/+ vs. Setd5^+/−, P < 0.0001). MITO area in soma, statistics, T-test (Setd5^+/+ vs. Setd5^+/−, P = 0.0003). MITO area in neurites, statistics, two-way ANOVA (row factor, P < 0.0001, column factor, P = 0.0004, subject, P = 0.088), multiple comparison Tuckey test (Setd5^+/+ vs. Setd5^+/−, 10 um, P = 0.9554, 20 um, P = 0.8030, 30 um, P = 0.2020, 40 um, P = 0.1782, 50 um, 0.0035) D Western blot quantification of mitochondrial fraction in synaptosomes, normalized on actin. Statistics, T-test (P = 0.0090)