Fig. 3
From: SETD5 haploinsufficiency affects mitochondrial compartment in neural cells
Impaired mitochondrial functionality in Setd5± NSCs. A Live imaging quantification of TMRM signals in both control and mutant NSCs during the time course of the experiment. Oligomycin and FCCP drugs were added at the indicated time points. Statistics, two-way ANOVA (P < 0.0001, for time, interaction and column factor), multiple comparison Bonferroni (Ctrl vs. Mut, 10′, P = 0.0003; 13, P = 0.0002; 16′, P = 0.0006; 20′, P < 0.0001; 23′, P < 0.0001; 26′, P < 0.0001; 30′, P < 0.0001; 33′, P < 0.0001; 36′, P < 0.0001; 40′, P < 0.0001; 46′, P < 0.0001; 50′, P = 0.1020; 53′, P = 0.0240; 56′, P > 0.9999; 60′, P = 0.2509; 63′, P > 0.9999; 66′, P = 0.0201) B–D Quantification by fluorescence of the ATP content (B), NAD + /NADH (C), and lactate (D) content in culture of both control and Setd5+/− NSCs (two different clones, C5 and D3). Statistics, ATP, one-way ANOVA (P = 0.077), multiple comparison Dunnet test (Ctrl vs. Clone C5, P = 0.0221; Ctrl vs. Clone D3, P = 0.0069). NAD/NADH, one-way ANOVA (P < 0.0001), multiple comparison Dunnet test (Ctrl vs. Clone C5, P = 0.0025; Ctrl vs. Clone D3, P < 0.0001). Lactate, one-way ANOVA (P < 0.0001), multiple comparison Dunnet test (Ctrl vs. Clone C5, P < 0.0001; Ctrl vs. Clone D3, P < 0.0001). E Bright-field images and growth curves of both control and mutant NSCs cultured either in presence of glucose in the media or without glucose and in presence of galactose as carbon source. Statistics, two-way ANOVA (interaction, P < 0.0001, time, P < 0.0001, column factor, P = 0.0069), multiple comparison Tuckey test (all non-significant, except, Ctrl. Gluc. vs. Mutant Gluc., P < 0.0001; Ctrl. Gal. vs. Mutant Gluc., P < 0.0001; Mutant Gluc. vs. Mutant Gal., P < 0.0001). All Data are presented as mean values+/− SEM