Fig. 3
From: Myt1l haploinsufficiency leads to obesity and multifaceted behavioral alterations in mice
No overt anatomical defects in the developing mouse brain of Myt1l mutant mice. A, B Sox2 staining (cyan) in E15.5 Myt1l (N = 3/genotype) shows no gross difference in VZ compared with littermate controls. C The number of Sox2-positive neurons was quantified as the percentage of Sox2-positive area in a 100 × 100 µm area in the ventricular zone. D Coronal sections stained for Tbr2 (green), Ctip2 (red), and counterstained with the nuclear stain DAPI (blue). E The size of layers expressing Tbr2 and Ctip2 remains similar between all three genotypes (N = 3/genotype). F The density of Ctip2 positive neurons was assessed by counting in a field of 50 × 100 µm in the central CP. G Nissl-stained whole brains from E18.5 embryos. H Myt1l mutants show similar gross brain anatomy, including the rostral and caudal cortex (motor cortex region M1, sensory cortex barrel region S1), corpus callosum, striatum, septum, hippocampus, thalamus, and hypothalamus, compared to WT and HET. N = 3/genotype. (Left and right hemispheres of one animal are indicated as arrowheads leading in the same direction; MZ marginal zone, CP cortical plate, IZ intermediate zone, (d)SVZ (distal) subventricular zone, MAZ multipolar cell accumulation zone, (p)VZ (proximal) ventricular zone, V ventricle)