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Table 1 Cohorts, number of animals and housing conditions for all behavior experiments

From: MEK inhibition ameliorates social behavior phenotypes in a Spred1 knockout mouse model for RASopathy disorders

Background strain

Sex

N

Behavior test

Housing

Figure

Neonatal mice

     

B6

Male

Female

WT n = 17; Spred1-/- n = 12

WT n = 7; Spred1-/- n = 10

USV at P4-12

Righting reflex P4, P8 (first subgroup only, 12–17/genotype)

With birth litter and dam

Figure 2, Additional file 1: Fig. 3A, B

B6

Spred1+/− Female dams for the above neonatal cohort

Naïve n = 7; experienced n = 5

Note: for experienced dams—2nd litter n = 3; 3rd litter n = 1; 4th litter n = 1

  

Adult mice

     

B6;129T2 F2

Female

Male

WT n = 8; Spred1-/- n = 8

WT n = 8; Spred1-/- n = 8

Tube test at 9–13 weeks

Couples: WT with Spred1-/-

Figure 1A, B

B6;129T2 F2

Male

Female

WT n = 6; Spred1-/- n = 7

WT n = 10; Spred1-/- n = 7

Open field at 6–15 weeks

Mixed genotype group housing

Additional file 1: Fig. 3C, D

B6

Male

WT n = 10; Spred1+/−  n = 10

Tube test at 10–15 weeks

Couples: WT with Spred1+/−

Additional file 1: Fig. 1D–F

B6

Female

WT n = 10; Spred1-/-  n = 10

Tube test at 11–20 weeks

nesting at 14–23 weeks

Couples: WT with Spred1-/-

Additional file 1: Fig. 1A–C

Figure 1G

B6

Female

WT n = 7; Spred1-/-  n = 9

Nesting at 20–35 weeks

Couples: WT with Spred1-/-

Figure 1G

B6

Female

WT n = 11; Spred1-/-  n = 11

Marble burying at 8–20 weeks

Mixed genotype group housing

Additional file 1: Fig. 2F

B6

Male

WT n = 15; Spred1-/- n = 14

Marble burying at 9–10 weeks (n = 8/genotype only)

USV at 10–11 weeks

Couples: WT with Spred1-/-

Additional file 1: Fig. 2F

Figure 1C–F

B6

Male

WT n = 9; Spred1-/- n = 9

Resident intruder at 10–13 weeks

Mixed genotype group housing

Additional file 1: Fig. 1G–H

B6

Female

WT n = 15; Spred1-/- n = 15

Three-chamber test at 20 weeks (n = 13–14/genotype)

burrowing at 28 weeks (n = 8/genotype only)

MEK inhibitor + nesting at 40 weeks. (WT + DMSO n = 8; WT + PD325901 n = 7; Spred1-/- + DMSO n = 7; Spred1-/- + PD325901 n = 8)

Mixed genotype group housing

Additional file 1: Fig. 2A–E

Additional file 1: Fig. 2G

Figure 4

MEK inhibitor + Automated tube test

     

B6: WT + DMSO vs Spred1-/- + DMSO

Male

WT + DMSO n = 4; Spred1-/- + DMSO n = 4

Tube test at 11–18 weeks

Couples: WT + DMSO with Spred1-/- + DMSO

Additional file 1: Fig. 4A–C

B6: WT + DMSO vs Spred1-/- + DMSO

Female

WT + DMSO n = 4; Spred1-/- + DMSO n = 4

Tube test at 10–11 weeks

Couples: WT with Spred1-/-

Additional file 1: Fig. 4D–F

B6: WT + DMSO vs Spred1-/- + PD325901

Female

WT + DMSO n = 8; Spred1-/- + PD325901 n = 8

Tube test at 10–12 weeks (cohort1), 23 weeks (cohort2)

Couples: WT + DMSO with Spred1-/- + PD325901

Figure 3A; Additional file 1: Fig. 5A

B6: WT + DMSO vs WT + PD325901

Female

WT + DMSO n = 8; WT + PD325901 n = 8

Tube test at 11–15 weeks

Couples: WT + DMSO with WT + PD325901

Figure 3C; Additional file 1: Fig. 5C

B6: Spred1-/- + DMSO vs Spred1-/- + PD325901

Male

Spred1-/- + DMSO n = 6; Spred1-/- + PD325901 n = 6

Tube test at 10–18 weeks

Couples: Spred1-/- + DMSO with Spred1-/- + PD325901

Figure 3B; Additional file 1: Fig. 5B