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Table 1 Cohorts, number of animals and housing conditions for all behavior experiments

From: MEK inhibition ameliorates social behavior phenotypes in a Spred1 knockout mouse model for RASopathy disorders

Background strain Sex N Behavior test Housing Figure
Neonatal mice      
B6 Male
Female
WT n = 17; Spred1-/- n = 12
WT n = 7; Spred1-/- n = 10
USV at P4-12
Righting reflex P4, P8 (first subgroup only, 12–17/genotype)
With birth litter and dam Figure 2, Additional file 1: Fig. 3A, B
B6 Spred1+/− Female dams for the above neonatal cohort Naïve n = 7; experienced n = 5 Note: for experienced dams—2nd litter n = 3; 3rd litter n = 1; 4th litter n = 1   
Adult mice      
B6;129T2 F2 Female
Male
WT n = 8; Spred1-/- n = 8
WT n = 8; Spred1-/- n = 8
Tube test at 9–13 weeks Couples: WT with Spred1-/- Figure 1A, B
B6;129T2 F2 Male
Female
WT n = 6; Spred1-/- n = 7
WT n = 10; Spred1-/- n = 7
Open field at 6–15 weeks Mixed genotype group housing Additional file 1: Fig. 3C, D
B6 Male WT n = 10; Spred1+/−  n = 10 Tube test at 10–15 weeks Couples: WT with Spred1+/− Additional file 1: Fig. 1D–F
B6 Female WT n = 10; Spred1-/-  n = 10 Tube test at 11–20 weeks
nesting at 14–23 weeks
Couples: WT with Spred1-/- Additional file 1: Fig. 1A–C
Figure 1G
B6 Female WT n = 7; Spred1-/-  n = 9 Nesting at 20–35 weeks Couples: WT with Spred1-/- Figure 1G
B6 Female WT n = 11; Spred1-/-  n = 11 Marble burying at 8–20 weeks Mixed genotype group housing Additional file 1: Fig. 2F
B6 Male WT n = 15; Spred1-/- n = 14 Marble burying at 9–10 weeks (n = 8/genotype only)
USV at 10–11 weeks
Couples: WT with Spred1-/- Additional file 1: Fig. 2F
Figure 1C–F
B6 Male WT n = 9; Spred1-/- n = 9 Resident intruder at 10–13 weeks Mixed genotype group housing Additional file 1: Fig. 1G–H
B6 Female WT n = 15; Spred1-/- n = 15 Three-chamber test at 20 weeks (n = 13–14/genotype)
burrowing at 28 weeks (n = 8/genotype only)
MEK inhibitor + nesting at 40 weeks. (WT + DMSO n = 8; WT + PD325901 n = 7; Spred1-/- + DMSO n = 7; Spred1-/- + PD325901 n = 8)
Mixed genotype group housing Additional file 1: Fig. 2A–E
Additional file 1: Fig. 2G
Figure 4
MEK inhibitor + Automated tube test      
B6: WT + DMSO vs Spred1-/- + DMSO Male WT + DMSO n = 4; Spred1-/- + DMSO n = 4 Tube test at 11–18 weeks Couples: WT + DMSO with Spred1-/- + DMSO Additional file 1: Fig. 4A–C
B6: WT + DMSO vs Spred1-/- + DMSO Female WT + DMSO n = 4; Spred1-/- + DMSO n = 4 Tube test at 10–11 weeks Couples: WT with Spred1-/- Additional file 1: Fig. 4D–F
B6: WT + DMSO vs Spred1-/- + PD325901 Female WT + DMSO n = 8; Spred1-/- + PD325901 n = 8 Tube test at 10–12 weeks (cohort1), 23 weeks (cohort2) Couples: WT + DMSO with Spred1-/- + PD325901 Figure 3A; Additional file 1: Fig. 5A
B6: WT + DMSO vs WT + PD325901 Female WT + DMSO n = 8; WT + PD325901 n = 8 Tube test at 11–15 weeks Couples: WT + DMSO with WT + PD325901 Figure 3C; Additional file 1: Fig. 5C
B6: Spred1-/- + DMSO vs Spred1-/- + PD325901 Male Spred1-/- + DMSO n = 6; Spred1-/- + PD325901 n = 6 Tube test at 10–18 weeks Couples: Spred1-/- + DMSO with Spred1-/- + PD325901 Figure 3B; Additional file 1: Fig. 5B