Background strain | Sex | N | Behavior test | Housing | Figure |
---|---|---|---|---|---|
Neonatal mice | Â | Â | Â | Â | Â |
B6 | Male Female | WT n = 17; Spred1-/- n = 12 WT n = 7; Spred1-/- n = 10 | USV at P4-12 Righting reflex P4, P8 (first subgroup only, 12–17/genotype) | With birth litter and dam | |
B6 | Spred1+/− Female dams for the above neonatal cohort | Naïve n = 7; experienced n = 5 | Note: for experienced dams—2nd litter n = 3; 3rd litter n = 1; 4th litter n = 1 |  |  |
Adult mice | Â | Â | Â | Â | Â |
B6;129T2 F2 | Female Male | WT n = 8; Spred1-/- n = 8 WT n = 8; Spred1-/- n = 8 | Tube test at 9–13 weeks | Couples: WT with Spred1-/- | Figure 1A, B |
B6;129T2 F2 | Male Female | WT n = 6; Spred1-/- n = 7 WT n = 10; Spred1-/- n = 7 | Open field at 6–15 weeks | Mixed genotype group housing | Additional file 1: Fig. 3C, D |
B6 | Male | WT n = 10; Spred1+/−  n = 10 | Tube test at 10–15 weeks | Couples: WT with Spred1+/− | Additional file 1: Fig. 1D–F |
B6 | Female | WT n = 10; Spred1-/-  n = 10 | Tube test at 11–20 weeks nesting at 14–23 weeks | Couples: WT with Spred1-/- | Additional file 1: Fig. 1A–C Figure 1G |
B6 | Female | WT n = 7; Spred1-/-  n = 9 | Nesting at 20–35 weeks | Couples: WT with Spred1-/- | Figure 1G |
B6 | Female | WT n = 11; Spred1-/-  n = 11 | Marble burying at 8–20 weeks | Mixed genotype group housing | Additional file 1: Fig. 2F |
B6 | Male | WT n = 15; Spred1-/- n = 14 | Marble burying at 9–10 weeks (n = 8/genotype only) USV at 10–11 weeks | Couples: WT with Spred1-/- | Additional file 1: Fig. 2F Figure 1C–F |
B6 | Male | WT n = 9; Spred1-/- n = 9 | Resident intruder at 10–13 weeks | Mixed genotype group housing | Additional file 1: Fig. 1G–H |
B6 | Female | WT n = 15; Spred1-/- n = 15 | Three-chamber test at 20 weeks (n = 13–14/genotype) burrowing at 28 weeks (n = 8/genotype only) MEK inhibitor + nesting at 40 weeks. (WT + DMSO n = 8; WT + PD325901 n = 7; Spred1-/- + DMSO n = 7; Spred1-/- + PD325901 n = 8) | Mixed genotype group housing | Additional file 1: Fig. 2A–E Additional file 1: Fig. 2G Figure 4 |
MEK inhibitor + Automated tube test |  |  |  |  |  |
B6: WT + DMSO vs Spred1-/- + DMSO | Male | WT + DMSO n = 4; Spred1-/- + DMSO n = 4 | Tube test at 11–18 weeks | Couples: WT + DMSO with Spred1-/- + DMSO | Additional file 1: Fig. 4A–C |
B6: WT + DMSO vs Spred1-/- + DMSO | Female | WT + DMSO n = 4; Spred1-/- + DMSO n = 4 | Tube test at 10–11 weeks | Couples: WT with Spred1-/- | Additional file 1: Fig. 4D–F |
B6: WT + DMSO vs Spred1-/- + PD325901 | Female | WT + DMSO n = 8; Spred1-/- + PD325901 n = 8 | Tube test at 10–12 weeks (cohort1), 23 weeks (cohort2) | Couples: WT + DMSO with Spred1-/- + PD325901 | |
B6: WT + DMSO vs WT + PD325901 | Female | WT + DMSO n = 8; WT + PD325901 n = 8 | Tube test at 11–15 weeks | Couples: WT + DMSO with WT + PD325901 | |
B6: Spred1-/- + DMSO vs Spred1-/- + PD325901 | Male | Spred1-/- + DMSO n = 6; Spred1-/- + PD325901 n = 6 | Tube test at 10–18 weeks | Couples: Spred1-/- + DMSO with Spred1-/- + PD325901 |