Fig. 2

Neonatal ultrasonic vocalization emissions, developmental growth, neurological reflexes and developmental delays in Arid1b^+/−. a Across neonatal developmental life, Arid1b^+/− pups displayed abnormal ultrasonic vocalization (USVs) emissions including delay of peak number of calls and decreased total number of USVs on several days compared to Arid1b^+/+. b When summed, Arid1b^+/− pups emitted significantly fewer USVs compared to Arid1b^+/+ littermate controls. c No genotype difference in axillary abdominal temperature was found, confirming fewer USVs were not the result of neonatal hypothermia. d Arid1b^+/− weighed less across development beginning at postnatal 8 and continuously throughout development compared to sex-matched Arid1b^+/+. e Across neonatal developmental life, Arid1b^+/− pups were shorter by body length (f) and narrower by head width measurements. g While Arid1b^+/− showed normal latencies to perform the righting reflex, a measure of limb coordination in early life, h Arid1b^+/− had prominent deficits in negative geotaxis (i.e., incline reorientation) and i the ability to traverse out of circle by walking. For a–c, Arid1b^+/+ N = 40, Arid1b^+/− N = 19, for d–i Arid1b^+/+ N = 29, Arid1b^+/+ N = 18. * p < .05 versus Arid1b^+/+ by repeated measures two-way ANOVA or student’s unpaired t test. Error bars represent mean ± SEM