Fig. 5

ALSM imaging of cleared hCSs. a Representative 3D volume image (600 × 600 × 600 µm) of cleared day 30 hCS immunostained with ZO-1 (green) and Sox2 (magenta) and imaged by ALSM. b Schematic image of ALSM optical sections of cleared hCS shown in c. c ALSM optical Sects. (600 × 600 × 50 µm) at 450, 500 and 550 µm depth, of cleared hCS shown in a. Cyan arrows indicate ZO-1-positive rosette lumen with surrounding radially organised Sox2-positive NPCs. Green and red bars indicate scale in X and Y planes (600 µm)—blue scale bar indicates scale in Z plane (600 µm in a and 50 µm in c)