Fig. 4

Spine density and morphology were similar in WT, Shank3-Het, and Shank3-KO. a Representative example of a deconvolved confocal image of a basal dendritic segment and b its 3D reconstruction, with the dendritic segment shown in light pink, thin spines in green, stubby in blue, mushroom in red, filopodia in yellow, and branched spines in magenta. Scale bar = 5 µm. c Total spine density and density by spine type d thin, e stubby, f mushroom, g filopodia, and h branched were comparable among the three genotypes. Note the difference in the scale of the Y-axes. WT, wild-type; Het, Shank3 heterozygotes; KO, Shank3 knockouts