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Fig. 3 | Molecular Autism

Fig. 3

From: High-throughput screening identifies histone deacetylase inhibitors that modulate GTF2I expression in 7q11.23 microduplication autism spectrum disorder patient-derived cortical neurons

Fig. 3

HTS workflow outline. a Compounds were tested at 10 μM for 48 h on NGN2 neurons seeded in 96-well plates. After RNA extraction and cDNA preparation, custom TaqMan Array 384-well plates were assembled through an automated TECAN Freedom EVO workstation. RT-qPCR were performed in QuantStudio™ 7 Flex Real-Time PCR System. b DAPI-stained (left) and GFP-positive (right) WBS01CN3 NGN2 neurons counted with Cellomics during differentiation. c Normalization panel for quantification of cell number (left) and GFP positive cells (right) in three different 96-well plates at DIV 28 (WBS01CN3 line). d Relative expression of BAZ1B, CLIP2, EIF4H, and GTF2I mRNA (mean ± SE) in day 28 WBS01CN3 neurons was measured by RT-qPCR, upon treatment with different DMSO concentrations. Highlighted in bold the DMSO concentration chosen for the screening

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Molecular Autism

ISSN: 2040-2392