Fig. 2

Dysregulation of TOP mRNAs in Tsc2^+/−. a Schematic depicting mTOR control of translation. mTOR controls mRNA-specific translation via 5′ terminal oligopyrimidine (TOP) motifs or long, structured 5′UTRs. mTOR controls global initiation and elongation via phosphorylation/dephosphorylation of 4E-BP, rpS6, and eEF2. b mRNA, RPF, and translation efficiency (TE) expression changes (Tsc2^+/−/WT log2FC) for TOP mRNAs (n = 68). Wilcoxon rank-sum test with correction by Bonferroni method. c Density plots of the hippocampal TE for the up and down RNA (left) and RPF (right) groups in Tsc2^+/−/WT compared to all genes (one-tailed Kolmogorov–Smirnov test). The hippocampal TE is calculated from the average of wild-type basal slices as the ratio of RPF to RNA. d Schematic depiction of how loss of Tsc2 could affect translation of lowly and highly translated mRNAs