Fig. 4

Dlg2^–/– mice display increased self-grooming behavior. Monitoring of behavior in LABORAS cages (home-cage activity) for 72 h showed that both the duration (a) and number (b) of bouts of self-grooming were significantly increased in Dlg2^–/– mice compared with WT animals. c, d Increased duration and number of bouts of self-grooming in Dlg2^–/– mice were found regardless of light on or off conditions, resulting in significant increases in total duration and number of bouts of self-grooming. WT, n = 10; Dlg2^–/–, n = 7. e Measurement of self-grooming behavior in an empty cage without bedding for 10 min showed that Dlg2^–/– mice also exhibited a significantly increased duration of self-grooming in this different setting. WT, n = 11; Dlg2^–/–, n = 12. Repeated measures of ANOVA was used for a and b, with post hoc Sidak’s multiple comparisons test applied under conditions where there was a significant effect of interaction (noted in a and b). Mann-Whitney tests were used for c–e. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001