Fig. 3

Characterization of iPSC-derived cIN NPCs. a Differentiation scheme, including timeline and small molecules used. b-d After 12 days of differentiation, cIN NPCs were stained with propidium iodide and analyzed by FACS for DNA content. (b) Representative FACS plots. In C and D, respectively, < 2 N (sub-G1) and 2 N (G1) cells were quantified, with values shown for each replicate (n = 3 or more biological replicates from each of two clonal lines for each subject, and one clonal line for the UC). e Mean percentages of cells in each cell cycle stage are shown (n = 3 or more biological replicates from each of two clonal lines for each subject). f-g cIN NPCs were plated in equal numbers for each sample and counted after four days of culture. Data are quantified in (f) and representative images are shown in (g) (n = 3 or more biological replicates from each of two clonal lines for each subject, and one clonal line for the UC). P values *P < 0.05, **P < 0.01, ***P < 0.001 were determined by one-way ANOVA and all other pairwise comparisons had a non-significant P value (P ≥ 0.05). Red and black data points denote experiments performed with clone 1 or with clone 2, respectively