Fig. 3
From: Increased Ca2+ signaling in NRXN1α+/− neurons derived from ASD induced pluripotent stem cells
Spontaneous calcium transients are voltage-gated calcium, action potential, and excitatory dependent. a Representative raster plots (activity of 100 cells in 300 s of recording, each dot represents Ca2+ transient activity, Line 4CX1), waterfall traces (3D representative of calcium transients in 300 s over defined threshold level, Line 4CX1), and their significant change showing the spontaneous Ca2+ transient activity of the cells and their response after the application of DL-AP5 (50 μM, a) and CNQX (50 μM, b), Nifedipine (50 μM, c), agatoxin (500 nM, d), and TTX (1 μM, e) (n = 2–3, 1CC1, 3VCX1, 4CX1). The red dotted line shows the point of drug application. All data summary are mean ± SEM