Fig. 1
From: Increased Ca2+ signaling in NRXN1α+/− neurons derived from ASD induced pluripotent stem cells
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging (c). iPSCs were characterized and were stained positive for alkaline phosphatase (d) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 (b–g). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h), ectoderm (TUJ1, i), and endoderm (AFP, j). iPSCs also showed positive expression of proliferating marker Ki67 (k) and (m) phase marker PH3 (l). They were also quantified at mRNA level for the expression of OCT4 (n) and SOX2 (o). All representative images all from control line 4CCX1