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Fig. 3 | Molecular Autism

Fig. 3

From: CYFIP1 overexpression increases fear response in mice but does not affect social or repetitive behavioral phenotypes

Fig. 3

CYFIP1-overexpressing mice show no anxiety, hyperactivity, or intellectual disability. a Anxiety was assessed in the elevated plus maze by calculating time spent in the open arms of the maze compared to the closed arms. Tg mice the same amounts of time in the open and closed arms as their WT littermates (Line 1: WT closed arm = 186.103 ± 5.485. WT open arm = 49.5 ± 4.101. Tg #1 closed arm = 180.326 ± 4.937, Tg #1 open arm = 47.011 ± 4.224. Line 2: WT closed arm = 195.417 ± 6.695. WT open arm = 57.506 ± 7.440. Tg #2 closed arm = 192.243 ± 9.277. Tg #2 open arm = 55.541 ± 8.862. b Anxiety was also assessed using the open field test. There was no increase in time spent near the edges of the field (surround) in the Tg mice compared to their WT littermates, demonstrating no increased anxiety mice (Line 1: WT center = 488.575 ± 26.44, surround = 711.425 ± 26.44. Tg #1 center = 450.895 ± 35.195, surround = 749.105 ± 35.195. Line 2: WT center = 51.344 ± 50.432, surround = 688.656 ± 50.432. Tg #2 center: = 411.978 ± 40.114, surround = 788.022 ± 40.114). c Hyperactivity was assessed using the open field test by measuring the distance traveled over 20 min. There was no difference in the distance traveled over time between the Tg and WT mice, indicating no hyperactivity. d Learning and spatial memory was assessed using the Morris water maze. Mice were trained on the location of the platform for 5 days. After training on day 5, mice were subject to a probe test where the platform was removed and time spent in each quadrant was measured. Next, the platform was moved to the opposite quadrant, and mice were trained for 5 additional days with a probe test at the end of day 10. e The average latency to platform discovery was recorded for each training day. Tg #2 mice showed a significant delay in learning the location of the platform on day 2 and 4 (left). This was not observed in Tg #1 mice (right). Neither Tg line showed reversal learning deficits. f Tg #2 but not Tg #1 mice spent significantly less time searching in the platform quadrant than their WT littermates during the first probe trial (Line 1: WT platform quadrant = 31.33 ± 2.78, empty quadrant = 22.89 ± 0.93. Tg #1 platform quadrant = 35.95 ± 2.50, empty quadrant = 21.35 ± 0.83. Line 2: WT platform quadrant = 34.28 ± 2.02, empty quadrant = 21.91 ± 0.67. Tg #2 platform quadrant = 26.23 ± 2.49, empty quadrant = 24.59 ± 0.83). g Tg mice performed just as well as controls during the probe trial and spent more time in the platform quadrant than the other quadrants (Line 1: WT platform quadrant = 40.00 ± 2.40, WT empty quadrant = 20.29 ± 0.82, WT old platform quadrant = 12.70 ± 1.86. Tg #1 platform quadrant = 39.08 ± 3.25, Tg #1 empty quadrant = 20.47 ± 1.11, Tg #1 old platform quadrant = 14.32 ± 1.85. Line 2: WT platform quadrant = 36.97 ± 2.09, WT empty quadrant = 21.27 ± 0.71, WT old platform quadrant = 13.41 ± 1.40. Tg #2 platform quadrant = 38.63 ± 3.25, Tg #2 empty quadrant = 20.56 ± 1.10, Tg #2 old platform quadrant = 13.00 ± 1.84). NS. not significant. *p < 0.05. See Table S3 for n’s

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