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Fig. 7 | Molecular Autism

Fig. 7

From: Autism-associated CHD8 deficiency impairs axon development and migration of cortical neurons

Fig. 7

Chd8 knockdown delays neuronal migration in vivo. a Representative images of coronal slices of E18.5 mouse brains that were transfected with GFP together with indicated Chd8 constructs by in utero electroporation at E14.5. At E18.5, cortical neurons transfected with Chd8-shRNA constructs were misplaced in the VZ/SVZ instead of CP. Transfected cells were visualized by staining coronal slices with GFP antibody. Scale bar, 200 μm. b Quantitative analysis of the distribution of cortical neurons transfected with different constructs across three different zones in the somatosensory cortex: upper cp, lower cp, VZ/SVZ. Results are mean ± SEM from 6 to 12 sections (one per mouse) for each experiment. c, d By P3, cortical neurons migrated normally from the SVZ into the cortex in both control and Chd8 RNAi-transfected brains. Scale bar, 120 μm. e, f At P7, most transfected neurons were located in the layers II/III in both control and Chd8 shRNA-transfected brains. Scale bar, 200 μm

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