Fig. 1

Generation and characterization of H9 hNSC overexpressing miR-146a. a Expression of miR-146a in the brain of ASD patients. Expression was measured in the temporal lobe of ASD patients (white box) and age-matched controls (gray box). Box plot showing relative expression of miR-146a measured by Taqman RT-qPCR for two house keeping miRNAs (miR-106a and miR-17) and the average of all controls. ***Fold change > 1.2 and P < 0.001 by Mann Whitney Test. b Phase contrast image showing morphology of hNSC lines in undifferentiated (day 0) and differentiated condition upon withdrawal of GF (days 7 and 14). c Expression of miR-146a (±S.D.) in H9 hNSC with stable construct compared to untransduced cells. Analyses were measured by Taqman assay, normalized against U6. d Expression of GFP transgene (±S.D.) in the two newly established hNSC H9 overexpressing miR-146a or miR-146a-Mut. Relative expression was measured by RT-qPCR, normalized against GPBP1. e Repression ratio (miR-146a/miR-146a mut) of known targets of miR-146a in undifferentiated H9 hNSC by RNA-Seq analyses. *P < 0.05, **P < 0.01, ***P > 0.001 by EdgeR analysis