Fig. 1

Proportion of hyper- and hypomethylated sites in the CpG and CpH contexts. Proportion of sites (y-axis) across increasingly stringent differentially methylated p value cutoffs (x-axis). The number of cytosines at each differentially methylated p value cutoff are displayed in the tables (below). a With approximately half of all sites demonstrating increased methylation (navy) and the other half decreased methylation (light blue), CpG sites behave as expected under the null. This pattern holds across increasingly stringent differential methylation p value cutoffs demonstrating no global differences in methylation within the CpG context. b The proportion of cytosines demonstrating hypermethylation is not significantly different from the proportion demonstrating hypomethylation when looking at all CpH sites; however, with increasingly stringent differentially methylated p value cutoffs, there is a significant proportion of hypermethylated CpH sites in the autistic brain