Phospho-S6/S6 ratios of lymphoblastoid cells from autistic individuals and their unaffected sibling from the SSC. ELISA analyses showed that one autistic individual had a significantly increased phospho-S6/S6 ratio compared to his healthy sibling (two-way ANOVA, family and disease status as fixed factors, disease status F(1,218) = 0.07, p = 0.79; family F(36,218) = 3.02, p < 0.0001; interaction F(36,218) = 1.24, p = 0.17; false discovery rate-corrected *p = 0.003). N = 3–4, shown are means + SEM. List of p values for all pairwise comparisons in Additional file 2: Table S1b. Further control experiments are described in Additional file 1: Figure S1d.