Fig. 1

Identification of a conserved miRNA signature in ASD. a Significantly deregulated miRNAs after validation in ASD OMSC after the validation round, representing the miRNA deregulation signature of ASD: miR-146a, miR-221, miR-654-5p, and miR-656 (±SD, controls—dark gray bar, patients—light gray bar). Dots represent average of technical triplicates for each biological repeat. *P < 0.05, **P < 0.01 by Wilcoxon rank sum test. b Tissue and disease specificity of miRNA signature. Expression (±SD) of miR-146a, miR-221, miR-654-5p, and miR-656 were assessed in primary skin fibroblasts of ASD patients (n = 5, light gray bar), ID patients (n = 12, white bar), and controls (n = 4, dark gray bar). Results were obtained from Taqman assays performed on Fluidigm array. Dots represent average of technical triplicates for each biological repeat. *P < 0.05, ***P < 0,001 by Student’s paired two-tailed t test. c Expression (±SD) of miRNA signature in peripheral blood mononuclear cells (PBMC). No significant difference in miR expression was detected between the controls (n = 20, dark gray box) and the ASD patients (n = 9, light gray box). Results were obtained from Taqman assays performed on Fluidigm array. Dots represent average of technical triplicates for each biological repeat