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Figure 3 | Molecular Autism

Figure 3

From: Phenotypic and functional analysis of SHANK3 stop mutations identified in individuals with ASD and/or ID

Figure 3

Overexpression of truncated SHANK3 variants results in distinct alterations of dendritic tree complexity. (A-D) Dendritic tree complexity of rat primary hippocampal neurons co-transfected with DenMark and either empty vector-based GFP (Control), full-length GFP-Myc-SHANK3 (SHANK3), or the truncated GFP-Myc-SHANK3 variants G1527A, 2497delG, and A5008T (DIV11-14). (A) Representative images of the DenMark signal in rat primary hippocampal neurons overexpressing either Control or SHANK3, as indicated. The panel on the right shows a Sholl analysis of Control (white-filled circles) vs. SHANK3 (black-filled circles). (B-D) Representative images of the DenMark signal in rat primary hippocampal neurons overexpressing either G1527A (B), 2497delG (C), or A5008T (D), as indicated. The panels below each image show Sholl analyses either of Control (white-filled circles, upper panel) or SHANK3 (black-filled circles, lower panel) vs. the corresponding truncated SHANK3 variant (red-filled circles for G1527A in (B), green-filled circles for 2497delG in (C), and blue-filled circles for A5008T in (D)). *P < 0.05, **P < 0.01, ***P < 0.001 compared with either Control or SHANK3.

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