Nuclear accumulation of truncated SHANK3 variants G1527A and 2497delG. (A-E) Subcellular distribution of empty vector-based GFP (Control) (A), full-length GFP-Myc-SHANK3 (SHANK3) (B), or the truncated GFP-Myc-SHANK3 variants G1527A (C), 2497delG (D), and A5008T (E), after transient overexpression (DIV11-14) in rat primary hippocampal neurons. All neurons shown were co-transfected with the DenMark construct  containing an mCherry sequence to demarcate dendrites and spines in red. They were further immunostained for VGLUT1 (not shown), and nuclei were visualized by DAPI. In each panel (A-E), one representative co-transfected neuron is depicted. The picture on the left is a merge of the GFP and DenMark signals, while the picture in the middle shows only the GFP signal. The upper insets on the right show a merge of GFP and DAPI signals or the DAPI signal alone, and the lower inset on the right shows a representative secondary dendrite as a merge of the GFP and DenMark signals. Note the strong overlap of both G1527A and 2497delG with the DAPI signal.