The accumulation of the mutated GPR85 (M152T) and GPR85 (V221L) in C2C5 cells. (a) Intracellular accumulates of wild-type and the two mutated GPR85. Green, full-length GPR85 (wild-type or mutants)-Myc-His; blue, Hoechst. (b) Percentages of cells showing accumulates were determined by counting cells expressing wild-type and mutated GPR85 at 28 h after transfection. Scale bars, 25 μm.