Decrease in mRNA levels of ERβ and CYP19A1, but no change in ERα mRNA in the middle frontal gyrus of ASD subjects. (A) No significant change in ERα mRNA. (B) Significant reduction in ERβ mRNA in the ASD subjects. (C) CYP19A1 mRNA levels were significantly lower in the ASD subjects. mRNA levels were determined by qRT-PCR, and the values were normalized to the geometric mean of two control genes (GAPDH and β-actin). ***P <0.001 vs. controls.