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Table 1 Identification of GST-N-SERT and GST-C-SERT pulled-down proteins from mouse brain extracts

From: N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism

Spot number Gene name Protein name MW (Da) Number Sequence coverage Score Accession number N-terminal specific Cellular and molecular events
N-1 Synj1 Synaptojanin 1 172,509 23 14% 785 Q8CHC4 Endocytosis
N-2 Cand1 Cullin-associated NEDD8-dissociated protein 1 136,245 14 10% 526 Q6ZQ38 SCF complex assembly
N-3 Aco2 Aconitate hydratase, mitochondrial 85,410 14 19% 534 Q99KI0   
N-4 Nsf Vesicle-fusing ATPase (NSF) 82,561 24 27% 1,010 P46460  
N-5 Atp6v1a V-type proton ATPase catalytic subunit A 68,283 13 21% 466 P50516 Hydrolysis
N-6 Crmp1 Dihydropyrimidinase-related protein 1 62,129 12 20% 441 P97427 Axon guidance and cell migration
N-7 Cct2 T-complex protein 1 subunit beta 57,441 11 19% 202 P80314 Molecular chaperone
N-8 Fscn1 Fascin 54,474 14 25% 174 Q61553 Actin filament binding
N-9 Eno1 Alpha-enolase 47,111 16 24% 703 P17182   
N-10 Cnp 2′,3′-cyclic-nucleotide 3′-phosphodiesterase 47,094 22 40% 341 P16330   
C-1 Aco2 Aconitate hydratase, mitochondrial 85,410 8 9% 287 Q99KI0   
C-2 Eno1 Alpha-enolase 47,111 8 20% 384 P17182   
C-3 Cnp 2′,3′-cyclic-nucleotide 3′-phosphodiesterase 47,094 18 32% 225 P16330   
  1. C-SERT, C-terminal domain of the serotonin transporter; GST, glutathione S-transferase; MW, molecular weight; N-SERT, N-terminal domain of the serotonin transporter.