Histopathologic characterization of the BTBR mouse model of autistic-like behavior reveals selective changes in neurodevelopmental proteins and adult hippocampal neurogenesis

Table 2 Results of histopathological changes in BTBR versus B6 mice

Marker	PFC	STR	ACC	AMG	HPC	Comments
AchE	0	0	0	0	0
BrdU	N/A	N/A	N/A	N/A	-
+ GFAP	N/A	N/A	N/A	N/A	+
+ NeuN	N/A	N/A	N/A	N/A	-
CNPase	0	0	+	0	0	Focal in CC region
Drebrin	0	0	-	0	0
Doublecortin	0	0	0	0	-
GAD65	0	0	-	0	0
GAD67	0	0	-	0	0
GFAP	0	0	0	0	0	Misorientation (alv., cing.)
GluR1	0	0	-	0	0
Iba1	0	0	0	0	0	Misorientation (alv., cing.)
MAP2	0	0	0	0	0
MBP	0	0	+	0	0	Focal in CC region
NeuroD	N/A	N/A	N/A	N/A	-
NG2	0	0	++	0	0
Parvalbumin	0	0	-	0	0
PSA-NCAM	0	0	0	0	-
PSD-95	0	0	-	0	0
Synapsin 2	0	0	-	0	0
Synaptophysin	0	0	-	0	0
Timm's	0	0	0	0	0
VGluT1	0	0	-	0	0

PFC = prefrontal cortex; STR = striatum; ACC = anterior cingulate cortex; AMG = amygdala; HPC = hippocampus; AchE = acetylcholinesterase; BrdU = 5-bromo-2'-deoxyuridine; CNPase = 2',3'-cyclic nucleotide 3'-phosphodiesterase; GAD65 = glutamic acid decarboxylase 65 kDa isoform; GAD67 = glutamic acid decarboxylase 67 kDa isoform; GFAP = glial fibrillary acidic protein; GluR1 = glutamate receptor 1; Iba1 = ionized calcium-binding adaptor molecule 1; MAP2 = microtubule-associated protein 2; MBP = myelin basic protein; N/A: not analyzed; NeuN = neuronal nuclei; PSA-NCAM = poly-sialic acid neural cell adhesion molecule; PSD-95 = postsynaptic density protein of 95 kDa; VGluT1 = vesicular glutamate transporter 1.
Comments refer to alterations probably related to callosal agenesis in the BTBR mice (-) = decreased staining compared with B6; (0) = equal staining compared with B6; (+) = moderately increased staining compared with B6; (++) = strongly increased staining compared with B6.

ISSN: 2040-2392