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Table 1 Real-time PCR primer and probe sequences for the pol xenotropic murine leukemia virus-related virus (XMRV) assay.

From: PCR and serology find no association between xenotropic murine leukemia virus-related virus (XMRV) and autism

Name

Sequence (5'→3')1

pol (2794 to 3062)

 

Primers

 

   Forward

GGGGATCAAGCCCCACATA

   Reverse

GGTGGAGTCTCAGGCAGAAAA

Probe

[6FAM] TGTTCCAGGGGGACTGGCAAGGTACCAccctgg [DABC]2,3

  1. 1Reference sequence was the VP62 XMRV strain (GenBank: EF185282.1).
  2. 2Lower case bases were added to form the stem.
  3. 3[6FAM] and [DABC] are the fluorophore FAM and the quencher Dabcyl, respectively.
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Molecular Autism

ISSN: 2040-2392