Spatial localisation in autism: evidence for differences in early cortical visual processing
© Latham et al.; licensee BioMed Central Ltd. 2013
Received: 21 September 2012
Accepted: 31 January 2013
Published: 19 February 2013
Vision in people with autism spectrum conditions (ASC) is reported to be different from people without ASC, but the neural level at which the differences begin to occur is not yet known. Here we examine two variants of a vernier acuity task to determine if differences are evident in early visual processing.
Abutting and separated vernier acuity was assessed in 16 people with ASC and 14 matched controls. In controls, abutting and separated thresholds were unrelated (r = 0.13, p = 0.65), suggesting thresholds are determined by two separate mechanisms. In contrast, the abutting and separated thresholds of ASC observers were strongly correlated (r = 0.88, p < 0.0001), with separated thresholds tending towards being superior to those of controls [t(28) = −2.46, p = 0.02].
The findings suggest the mechanisms employed by ASC observers in separated vernier tasks are different to those of controls. This psychophysical evidence suggests that visual differences in ASC may begin at an early cortical stage of visual processing.
KeywordsAutism spectrum conditions Spatial vision Vernier acuity Hyperacuity Psychophysics Visual processing
Where in the visual processing system do differences in vision begin to arise for people with autistic spectrum conditions (ASC)? People with ASC appear to have normal responses to basic visual tasks , but difficulties with complex face recognition tasks and enhanced attention to local visual information over global in tasks such as visual search [2, 3]. To understand the nature and location of differences in visual processing in ASC, Simmons et al.  state: “A really basic characterization of the visual processing capabilities of people with ASC would be extremely useful, even if all it could do was say with certainty ‘nothing is wrong here’”.
Starting with basic visual processing, visual acuity (or the minimum recognisable acuity) is limited by foveal cone spacing  and is similar in people with ASC and controls . Moving methodically up the visual pathway, the next logical visual function to examine is the spatial localisation of two features, or the minimum discriminable acuity. Localisation thresholds can be more precise than cone spacing and are limited in the primary visual cortex [5–8].
Here, we assess spatial localisation using a vernier acuity paradigm across two conditions that are thought to be mediated by different neural mechanisms: (1) abutting line vernier targets, processed by contrast-dependent spatial filters encompassing both stimulus elements [5, 6]; (2) line vernier targets separated by 10 arc min. For lines separated by more than 4 arc min [5, 6], vernier thresholds are contrast-independent, the mechanism underlying performance involving position identification of each stimulus element using a local sign process , followed by a collator mechanism capable of comparing the responses to the two individual stimulus elements [6–8]. Our hypothesis, based on earlier evidence, is that spatial localisation in ASC and control observers will be similar, with differences arising at higher (attentional) levels of visual processing.
The position of the test stimulus presentation was jittered between trials to prevent the observers from making judgements based on the absolute position of the test line with respect to other cues such as the edge of the monitor display and was presented until a response was made. Response times were recorded and no feedback was given. Initially a practice session was undertaken with three trials per block to check that step sizes covered the range from approximately 0-100% rightward responses and to familiarise the observer with the task and response keys. This was repeated if necessary to confirm step size requirements. The 105 trials in each block took approximately 2–5 min to complete. Four blocks were completed for each observer: half in the order AGGA and half in the order GAAG (where A is the abutting condition and G the condition separated by a gap).
For each block of trials, the number of rightward responses was tallied for each offset position. A cumulative Gaussian function was used to fit the data, from which we derived two important parameters: (1) the offset from the zero-offset condition (the two lines were perfectly aligned) that corresponded to 50%-rightward response, representing the response bias, and (2) the offset between the two lines to change the rightward response on the psychometric function from 50 to 84%, representing the vernier threshold. This definition of vernier threshold is equivalent to 1 SD of the cumulative Gaussian function that was fitted to the observer’s responses.
The authors conducting the experiments and analysing the data were blind to whether participants were in the ASC or control groups until after data had been collected and analysed.
Participants were included if they met the following inclusion criteria: aged over 18 years, an Intelligence Quotient [IQ: Wechsler Abbreviated Scale of Intelligence (WASI)] of at least 90 so as to exclude those with ‘low average’ IQ or below , no self-reported cognitive co-morbidities, corrected habitual binocular visual acuity at least 0.00 logMAR and no manifest strabismus as assessed by optometric screening. In addition, participants with ASC had a clinical diagnosis based on DSM-IV criteria and an Autism Spectrum Quotient (AQ) score of at least 26 . Controls had an AQ score of less than 22. Ethical approval for the study was obtained from Anglia Ruskin University, and informed consent was obtained from all participants.
ASC (n= 16)
Controls (n= 14)
Two-tailed independent t-test (Levene > 0.05; equal variances assumed)
Mean ± SD (range)
Mean ± SD (range)
34.4±9.8 years (20–54 years)
38.1 ± 6.3 years (26–48 years)
t = 1.19, df28, p = 0.24
9 female, 7 male
6 female, 8 male
Pearson chi-square 0.54, p = 0.46
120.5 ± 10.9 (99–135)
115.5 ± 9.4 (100–130)
t = 1.30, df26, p = 0.20
40.7 ± 4.6 (29–48)
12.4 ± 4.3 (4–21)
t =16.6, df28, p < 0.0001
−0.15 ± 0.09 logMAR (0.00 - -0.28)
−0.16 ± 0.10 logMAR (0.00 - -0.30)
t = 0.27 df27 p = 0.79
There was no difference in response bias for abutting stimuli [ASC −2.5 ± 4.7, control −2.2 ± 5.7; t(28)-1.15, p = 0.88] or for separated stimuli [ASC −3.2 ± 8.8, control +3.4 ± 9.0; t(28)-2.01, p = 0.06].
Group-mean vernier thresholds (± SD) in log sec arc, taken as the geometric mean of two blocks of responses for each participant
1.16 ± 0.20
1.21 ± 0.20
1.35 ± 0.20
1.52 ± 0.15
However, of greater interest than the mean difference between the groups is the relationship between abutting and separated vernier thresholds for the two groups as shown in Figure 1. In controls (filled points), the abutting and separated thresholds are unrelated (Pearson r = 0.13, p = 0.65). In ASC (open points), the abutting and separated thresholds rise in proportion to one another (Pearson r = 0.88, p < 0.0001).
It would be expected that abutting and separated vernier thresholds would be unrelated, since in typical controls they have been shown to be determined by two separate mechanisms. As previously outlined, abutting thresholds are determined by contrast-dependent spatial filters encompassing both elements [5, 6], whilst separated thresholds are determined by a two-step process of local sign designation followed by comparison of features [6–8]. The results of the control observers are consistent with these findings.
The finding of a strong correlation between abutting and separated vernier thresholds in the ASC observers suggests that their thresholds for the two conditions may be determined by the same mechanism. People with ASC may have spatial filters that act over greater separations than in control observers, such that they do not need to switch to a comparison mechanism at a 10 arc min separation. However, it is not clear how such larger spatial filters would have the capacity to retain a high accuracy of vernier judgement. Future work to test this hypothesis might include an exploration of the influence of contrast , reverse polarity elements  or spatial frequency masks  on the separated vernier thresholds of observers with ASC.
The finding of differences in ASC in the mechanisms used to determine vernier thresholds is not consistent with visual differences in autism being solely due to changes in higher level attention rather than early sensory processing [2, 14], nor are the findings consistent with the suggestion that visual thresholds for tasks with two stages of processing, such as the separated vernier task, are poorer in ASC because of atypical cortical lateral connectivity . Neuroanatomical studies suggest high local connectivity between smaller minicolumns in the autistic primary visual cortex  that might be implicated in differences in threshold mechanisms in people with ASC, and atypical lateral connectivity has also been suggested to explain psychophysical findings of better contrast detection in the presence of lateral masks in ASC . Enhanced performance in ASC has been shown for visual simultaneity thresholds , a localisation task in the temporal domain, which is similar to the task reported here in the spatial domain.
In conclusion, we show that vernier thresholds for abutting and separated vernier stimuli are not related in controls but are strongly correlated in ASC, suggesting that people with ASC employ different mechanisms than controls in processing separated vernier stimuli. These findings provide psychophysical evidence of a difference in visual processing in ASC at an early stage of cortical visual processing and demonstrate that visual changes in ASC are not restricted to higher level perceptual processes.
Autism spectrum conditions
Autism Spectrum Quotient
Wechsler Abbreviated Scale of Intelligence
We thank the participants and Dennis Levi and Caroline Robertson for helpful comments. TT was supported by the Pinsent Darwin Trust and Autistica, and SBC by the MRC UK and the Wellcome Trust. The study was conducted in association with the NIHR CLAHRC for Cambridgeshire and Peterborough NHS Trust.
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