Fig. 5
From: Shank3 deficiency elicits autistic-like behaviors by activating p38α in hypothalamic AgRP neurons
Inactivated p38α in AgRP neurons ameliorates the autistic-like behaviors of Shank3-/- mice. A. scheme of generating Shank3-/-:p38αAgRP-180/182 mice and immunofluorescence staining of p-p38α(green, 488 nm, FITC). B and C, the groom time and bouts in 24 h of Shank3-/-:p38αAgRP-con and Shank3-/-:p38αAgRP-180/182 mice. D. the total distance in 24 h of Shank3-/-:p38αAgRP-con and Shank3-/-:p38αAgRP-180/182 mice. E. the activity time in 24 h of Shank3-/-:p38αAgRP-con and Shank3-/-:p38αAgRP-180/182 mice. F. the first phase of the three-chamber test, the time of Shank3-/-:p38αAgRP-con and Shank3-/-:p38αAgRP-180/182 mice in stranger1 chamber. G. the second phase of the three-chamber test, the time of Shank3-/-:p38αAgRP-con and Shank3-/-:p38αAgRP-180/182 mice in stranger2 chamber. Shank3-/-:p38αAgRP-con and Shank3-/-:p38αAgRP-180/182 male mice were subjected to Home-Cage monitoring test at the age of 8 weeks, and subjected to three-chamber test at the age of 14 weeks, 12 mice in Shank3-/-:p38αAgRP-con and 19 mice in Shank3-/-:p38αAgRP-180/182 group. Statistical analysis: data were analyzed using unpaired two-tailed Student’s t-test (Prism9, GraphPad Software Inc.). Data were represented as Mean ± SD. Significance levels are indicated with *p < 0.05.