Table 2 Variants predicted to compromise TRIO-RAC1 stability (NCBI Reference Sequence for Trio: NM_007118.4)
Prediction | Variant | Base replacement | Effect on stability | ∆∆Gstability (kCal/mol) | ∆∆Gbinding (kCal/mol) | Probability of variant [38] |
|---|---|---|---|---|---|---|
Deleterious | E1304G | GAA→GGA | Disrupts H-bonds to R1428 and H1351, Y1432 | 3.92 | 1.00 | 0.01115 |
Deleterious | Y1318G | TAC→GGC | Distorts a-helix conformation, Disrupts H-bond to N1416 backbone | 5.46 | 1.02 | 0.00094 |
Deleterious | Y1383A | TAT→GCT | Disrupts H-bonds to H1351 and Y1307 | 3.83 | 0.98 | 0.00163 |
Deleterious | G1453W | GGC→TGG | Small to big in hydrophobic core | 3.94 | 4.57 | 0.00065 |
Benign | S1403F | TCC→TTC | TRIO surface, DH1 domain | 1.45 | 0.97 | 0.00750 |