Table 1 Variants predicted to compromise TRIO-RAC1 binding interface (NCBI Reference Sequence for Trio: NM_007118.4)
Prediction | Variant | Base replacement | Effect on binding | ∆∆Gstability (kCal/mol) | ∆∆Gbinding (kCal/mol) | Probability of variant [38] |
|---|---|---|---|---|---|---|
Deleterious | E1299W | GAG→TGG | Disrupts H-bond with Y32 and T35 and V36 backbone | 0.46 | 4.24 | 0.00037 |
Deleterious | C1387W | TGC→TGG | Bulky residue instead of small polar on the interaction interface | −1.71 | 16.22 | 0.00388 |
Deleterious | T1430W | ACG→TGG | Bulky residue instead of small polar on the interaction interface | −1.06 | 6.82 | 0.00076 |
Deleterious | A1464W | GCC→TGG | Bulky residue instead of small hydrophobic on the interaction interface | −0.12 | 14.12 | 0.00041 |
Benign | T1394A | ACT→GCT | TRIO-RAC1 binding interface, DH1 domain | 0.55 | 1.90 | 0.03483 |