Fig. 8

Characterization of Shank3B- mice in basal state. A Basal body temperature at 8am, as measured by subcutaneously implanted transponder (°C). B Spontaneous behaviour as measured by marbles buried in 20 min. C Description of the repetitive behaviours found in Shank3B- strain: grooming test for 5 min. D Assessment of the motor coordination and muscular strength on the horizontal bar test for 1 min (animals score 1 if they fall off within 10 s, score 2 if they hold on for 11–59 s, score 3 if they hold on for 60 s or reached the safe platform in 60 s, score 4 if they reached the safe platform within 30 s and score 5 if they reached the platform within 10 s. E Analyses of risk assessment and anxiety-like behaviours using the elevated zero maze (EZM) test and monitoring the latency time to go to the open zone of the maze, the number of events of risk assessment (neck, two paws and total events emerging) and the time spent in the open zone of the EZM during 5 min. F General and exploratory activity as assessed in the open-field test, showing the mean speed (m/s), number of rotations, time rearing (s) and travelled distance (m) for 10 min. G Social behaviour characterization using the frequency of sniffs and the time spent sniffing of a mouse in comparison with an inanimate object (ratio mouse:object) for 10 min. All data are expressed as mean ± SEM, n = 23 per group. * versus C57 strain by unpaired two-tailed t-test or Mann–Whitney, p < 0.05