Fig. 3

Differential patterns of labelling with cFOS. Immunohistochemistry for cFOS was performed in free-floating sections from brains of mice exposed to room temperature (RT), LPS (250 µg/kg) or whole-body hyperthermia (WBH) and quantitatively assessed (RT n = 7; LPS n = 8; WBH n = 6). Regions of interest are defined by their position from Bregma on the anterior–posterior axis. Abbreviations: PVN: paraventricular nucleus (− 0.58 to − 0.94 mm), LHab: lateral habenula (− 1.58 to − 2.06 mm), CeA: the central nucleus of the amygdala (− 1.58 to − 1.94 mm), PVT: paraventricular thalamus (− 1.46 to − 2.06 mm) MPO: medial preoptic nucleus (0.14–0.5 mm), DMH: dorsomedial hypothalamic nucleus (− 1.34 to − 1.94 mm) and LSV: lateral septum, ventral portion (0.3–0.76 mm). cFOS positive neurons were counted in the regions of interest, which are defined by the shapes indicated. Data are represented as mean ± SEM and analysed by one-way ANOVA where normally distributed and by median ± IQR and Kruskal–Wallis analysis when not normally distributed. After significant group analysis pairwise comparisons between LPS and RT are indicated by ^, between WBH and RT by * and between WBH and LPS by #. For all 3 symbols p values of 0.05, 0.01, 0.001 and 0.0001 are denoted by 1, 2, 3 or 4 repeats, respectively, of these symbols