Fig. 6

Ganaxolone pharmacological treatment rescues the behavioral alteration in Pv-Cre^+/− Shank3 ^Fl/Wt mice. A Repetitive behavior was evaluated as the time spent doing grooming. Grooming was analyzed by two-way ANOVA; n = 7 Pv-Cre^+/− Shank3^Wt/Wt vehicle, n = 8 Pv-Cre^+/− Shank3^Wt/Wt ganaxolone, n = 9 Pv-Cre^+/− Shank3^Fl/Wt vehicle, n = 10 Pv-Cre^+/− Shank3^Fl/Wt ganaxolone; * p < 0.05 Pv-Cre^+/− Shank3^Wt/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt vehicle. B Ganaxolone treatment rescues the novel object recognition memory of Pv-Cre^+/− Shank3^Fl/Wt mice. Novel object was analyzed by two-way ANOVA; n = 6 Pv-Cre^+/− Shank3^Wt/Wt vehicle, n = 7 Pv-Cre^+/− Shank3^Wt/Wt ganaxolone, n = 8 Pv-Cre^+/− Shank3^Fl/Wt vehicle, n = 10 Pv-Cre^+/− Shank3^Fl/Wt ganaxolone; *** p < 0.001, **** p < 0.0001 Pv-Cre^+/− Shank3^Wt/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt vehicle; $$$ p < 0.001; $$$$ p < 0.0001 Pv-Cre^+/− Shank3^Fl/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt ganaxolone. C Spatial memory was evaluated by determining a discrimination index in the spatial object recognition test. Data were analyzed by two-way ANOVA; n = 6 for each group; *** p < 0.001, **** p < 0.0001 Pv-Cre^+/− Shank3^Wt/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt vehicle; $$$$ p < 0.0001 Pv-Cre^+/− Shank3^Fl/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt ganaxolone. D Motor coordination was evaluated as the time spent to cross the beam with a flat surface of 12 or 6 mm width. All p-values were derived using two-way ANOVA; n = 6 for each group in the 12 mm test; n = 7 Pv-Cre^+/− Shank3^Wt/Wt vehicle, n = 7 Pv-Cre^+/− Shank3^Wt/Wt ganaxolone, n = 7 Pv-Cre^+/− Shank3^Fl/Wt vehicle, n = 6 Pv-Cre^+/− Shank3^Fl/Wt ganaxolone in the 6 mm balance test; ** p < 0.01, **** p < 0.0001 Pv-Cre^+/− Shank3^Wt/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt vehicle; $ p < 0.05; $$$$ p < 0.0001 Pv-Cre^+/− Shank3^Fl/Wt vehicle compared with Pv-Cre^+/− Shank3^Fl/Wt ganaxolone