Fig. 4

E/I balance is altered toward excitation when Shank3 is selectively disrupted in PV neurons even if in heterozygosis. A Spectral power analysis of Pv-Cre^+/− Shank3^Wt/Wt (N = 14 mice), Shank3 KO (N = 11 mice) and Pv-Cre^+/− Shank3^Fl/Wt mice (N = 10 mice). Spectral differences between Shank3 KO and Pv-Cre^+/− Shank3^Wt/Wt (indicated as KO-Wt in light red), and Pv-Cre^+/− Shank3^Fl/Wt and Pv-Cre^+/− Shank3^Wt/Wt (indicated as Pv-Wt in light blue) show a distinct E/I behavior in the two models, even in the direction of a more epileptic phenotype. B, C Graphs show an alteration of the spectral power for power less than 0.5 Hz (B) or frequencies larger than 100 Hz (C) (one-way ANOVA; *p < 0.05). D Exemplificative waveforms of slow-wave activity in control (black), Shank3 KO (red) and Pv-Cre^+/− Shank3^Fl/Wt (blue) anesthetized mice in which it appears evident the difference in US and DS modulation. * indicates a large transient suggestive of hypersynchronicity. Scale bar 0.4 mV, 1 s. E, F Shank3 mutation in PV neurons activity doesn’t affect US duration or frequency. G–I Shank3 mutation in PV neurons activity specifically affects US amplitude (G) and US power in HF band (I); one-way ANOVA, *p < 0.05), while doesn’t not affect US 25-80 Hz gamma power differently from what happens in Shank3 KO mice (see Fig. 1A)