Fig. 3
Shank3 haploinsufficiency specifically in inhibitory PV neurons is sufficient to induce behavioral deficits. A Increased repetitive grooming behavior in Pv-Cre+/− Shank3Fl/Wt compared to Pv-Cre+/− Shank3Wt/Wt mice. Grooming time was analyzed by unpaired, two-tailed Student’s t-test; n = 9 for each group; **p < 0.01. B Pv-Cre+/− Shank3Fl/Wt mice show an impairment in the novel object recognition test. Novel object recognition at 5 min was analyzed by unpaired, two-tailed Student’s t-test; novel object recognition at 120 min was analyzed by two-tailed Mann–Whitney test; Novel object recognition at 24 h was analyzed by unpaired, two-tailed Student’s t-test; n = 7 Pv-Cre+/− Shank3Wt/Wt, n = 8 Pv-Cre+/− Shank3Fl/Wt; **p < 0.01; ***p < 0.001. C Spatial memory was evaluated by determining a discrimination index in the spatial object recognition test. Pv-Cre+/− Shank3Fl/Wt mice have an impairment in the discrimination index in all the time point evaluated. Spatial object recognition at 5 min, 120 min and 24 h were analyzed by unpaired, two-tailed Student’s t-test; n = 7 for each group; **p < 0.01; ***p < 0.001. D Pv-Cre+/− Shank3Fl/Wt mice show impaired motor coordination in the balance beam test. Time to cross the 12 mm width beam was evaluated by unpaired, two-tailed Student’s t-test; time to cross the 6 mm width beam was analyzed by two-tailed Mann–Whitney test; n = 6 for each group; *p < 0.05. E Pv-Cre+/− Shank3Fl/Wt are impaired in the rotarod test. Unpaired, two-tailed Student’s t-test with Welch’s correction was used for the statistical analysis; n = 16 Pv-Cre+/− Shank3Wt/Wt, n = 17 Pv-Cre+/− Shank3Fl/Wt; *p < 0.05. F Pole test analysis show no alteration in Pv-Cre+/− Shank3Fl/Wt mice. Data were analyzed by unpaired, two-tailed Student’s t-test; n = 6 for each group. G Pv-Cre+/− Shank3Fl/Wt mice show normal muscle strength. Wire hanging test was evaluated by unpaired, two-tailed Student’s t-test; n = 6 for each group. H Social interaction was evaluated by the three-chamber assays. Unpaired, two-tailed Student’s t-test was used for statistical analysis; n = 11 Pv-Cre+/− Shank3Wt/Wt, n = 14 Pv-Cre+/− Shank3Fl/Wt