Fig. 2

Generation of mouse model and analysis of Parvalbumin expression. A Right, schematic strategy for the generation of Pv-Cre^+/− TdTomato^Fl/− Shank3^Fl/Wt mice that express the gene reporter TdTomato specifically in PV-positive cells. Left, representative images of PCR genotype analysis. B Hippocampal fluorescence images of Pv-Cre^+/− TdTomato^Fl/− Shank3^Wt/Wt mice at different ages. Parvalbumin expression (red) begins from 14 days after birth. C Representative images of Pv (green) and TdTomato (red) colocalization in hippocampal slice. D Quantification (left) and representative images (right) of Shank3 expression (green) in PV-positive neurons in CA1 stratum radiatum and visual cortex of Pv-Cre ^+/− Shank3^Wt/Wt and Pv-Cre ^+/− Shank3^Wt/Fl mice. Experiment in the CA1 stratum radiatum was analyzed by unpaired, two-tailed Student’s t-test; Visual cortex was analyzed by unpaired, two-tailed Student’s t-test with Welch’s correction; n = 5 Pv-Cre ^+/− Shank3^Wt/Wt, n = 5 Pv-Cre ^+/− Shank3^Fl/Wt; *p < 0.05; ****p < 0.0001. CA1 = Cornu Ammonis-1. Scale bar = 10 µm